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規格

規格	96 tests、480 tests、48 tests、192 tests
樣本類型	Cell Culture Supernatants, Serum
原理方法	Sandwich-based
檢測方法	Flow Cytometry
定量與否	Quantitative
載體	Fluorescent Microbeads
適用種屬	Human
檢測靶標	IFN-gamma, IL-1 beta, TNF alpha, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-4, GM-CSF, IL-6, IL-5, IL-22, IL-33
靶標數	14

詳細描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 μl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

6-plex panel: IL-2, IL-4, IL-6, IL-10, TNF alpha, IFN-gamma

7-plex panel: IL-2, IL-4, IL-6, IL-10, IL-17A, TNF alpha, IFN-gamma

14-plex panel: IL-1 beta (IL-1 F2), IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-15, IL-17A, IL-22, IL-33, GM-CSF, TNF alpha, IFN-gamma

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex? Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
RayBio? Serum Diluent

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex? bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex? Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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文件

FAH-TH.pdf

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