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規格

規格	4 樣本、8 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	Growth Hormone, bad, Bcl-w, BID, Cytochrome C, alpha-Synuclein, DAN, FSH, GRP, 14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, ABAT, ABCF1, ABI3BP, ACAA, ACAA1, ACAA2, ACACA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK, Ahsp, AIF, AK2, AKAP9, AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1, ALDH1A1, ALDH9A1, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha Tubulin, AlphaA Crystallin, ALS, ALS+B62:B1212CR1, Als2, Aminopeptidase??PILS, Androgen Receptor, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, ANP, APA, APM2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, ARX, ASH2L, Asialoglycoprotein Receptor 2, ASK1, Aspartate Aminotransferase, Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, Band 3, BASP1, Bassoon, BAZ2B, BCHE, BCOR, beta 1 Spectrin, beta B1 Crystallin, BIN2, BIRC6, BLMH, BLVRB, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C3orf75, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150, CA2, CA3, CACNB4, CAD, Cadherin-22, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1, Calpastatin, Calretinin, Calumenin, CAP1, CapG, CAPZA1, Carboxylesterase??1, Carboxypeptidase??E, CARHSP1, Caspase-14, Catalase, Catenin gamma, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD16, CD32, CD35, CD41, CD5L, CD9, CD98, CDA, CDC5L, CDK2, CEACAM-8, CECR1, CENPF, CEP57, CETP, Cezanne, CFHR 1, CFHR4, CFI, CFL1, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, CLPS, CLTA, CNN2, CNOT1, CO4A2, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, COG4, COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Complement Factor B, COPS8, Corneodesmosin, Coronin 3, Cortactin, COTL1, CPEB3, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin B, Cyclophilin??A, Cystatin D, Cystatin E/M, Cystatin SN, Cystatin??S, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin, DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, ECHS1, ECM-1, EEF1G, EEF2, EFEMP2, EFTUD2, EHD1, EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1, EMILIN1, EMSY, EN2, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, F-box protein 38, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, FBPase 1, FBPase 2, FCGBP, FDPS, FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP, G6PD, GALNT2, GAPHD, GARNL1, GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC, GCSH, GDA, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLPH4, GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GSTM1, GSTO1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, hHR23b, HIBADH, HINT1, HIP1R, Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha, hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G, hnRNP L, hnRNP M1-M4, hnRNP U, Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRG, HPRT, HRSP12, HSC70, HSP47, LAMR1, Tubulin beta-1 chain, Tubulin beta-3 chain, Ubiquitin-activating enzyme E1, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Cathepsin Z / Cathepsin X / Cathepsin P, Coagulation Factor VII / F7, Coagulation Factor XI / F11 / PTA, Carboxypeptidase M / CPM, DEP-1 / CD148, Human Amyloid-like protein 1 / APLP-1, Apolipoprotein(a), B7-H3 / CD276, Cadherin-6 / KCAD, Collectin-12 / CL-P1, Contactin-3 / BIG-1, FABP5 / E-FABP, CD155 / PVR, Aminoacylase-1 / ACY1, Alkaline Phosphatase / ALPL, Annexin A5 / Annexin V, Aminopeptidase LRAP / ERAP2, Antithrombin 3 / Serpin C1, B7-H2 / ICOS Ligand, C1S / Complement??Component??C1s, C4.4A / LYPD3, Carboxypeptidase B2 / CPB2, CD21 / CR2 / EBV receptor, CD39L4 / ENTPD5, CD42b / GPIb-alpha, CD48 / SLAMF2, Complement Factor H-related 5 / CFHR5, Cytokeratin 20 / KRT20, Endorepellin / Perlecan, GOLM1 / GP73, GPV / CD42d, Thrombospondin 5 / COMP, ADAM DEC1, Angiopoietin-like protein 6, Angiopoietin-like protein 8, BMP-1 / PCP, GDF-7 / BMP-12, Apolipoprotein A-I-binding protein / APOA1BP, Apolipoprotein F / ApoF, Apolipoprotein L1 / ApoL1, Apolipoprotein L2 / ApoL2
靶標數	500

詳細描述

Introduction

Recent technological advances by RayBiotech have enabled the largest commercially available glycosylation antibody array to date. With the Human Glycosylation Array L3, researchers can now efficiently obtain glycosylation profiles in their samples. The glycosylation profiles of 500 human proteins can be simultaneously detected, including, but not limited to, cytokines, chemokines, adipokines, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules in cell culture supernatants, serum, plasma and other samples types.

Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.

Product Features

High density screening
High sensitivity and specificity
Low sample volume (20-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Accurate and reproducible

Target Names

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, LAMR1, ABAT, ABCF1, ABI3BP, ACAA1, ACAA2, ACACA, ACAA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAMDEC1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK?, Ahsp, AIF, AK2?, AKAP9?, AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1?, ALDH1A1, ALDH9A1, Alkaline Phosphatase, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha-synuclein, alpha Tubulin, AlphaA Crystallin, ALS, Als2?, ALS+B62:B1212CR1?, Aminoacylase-1, Androgen Receptor, ANGPTL6, ANGPTL8, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, Annexin V, ANP, Antithrombin 3, APA, APLP-1, APM2, Apo (a), APOA1BP, ApoF, ApoL1, ApoL2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, Aminopeptidase?PILS, ARX, ASH2L?, Asialoglycoprotein Receptor 2, ASK1?, Aspartate Aminotransferase , Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, B7-H2, B7-H3, bad, Band 3, BASP1, Bassoon, BAZ2B, BCHE, bcl-w, BCOR, beta 1 Spectrin?, beta B1 Crystallin, Tubulin beta-1 chain, Tubulin beta-3 chain, BID, BIN2, BIRC6, BLMH, BLVRB, BMP-1, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C1S, C3orf75, C4.4A, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150?, CA2, CA3, CACNB4, CAD?, Cadherin-22, Cadherin-6, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1?, Calpastatin, Calretinin, Calumenin?, CAP1, CapG, CAPZA1, Carboxypeptidase B2, CARHSP1, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, Cathepsin Z, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD155, ADP-Ribosyl Cyclase 2, CD16, CD21, CD32, CD35, CD39L4, CD41, CD42b, CD48, CD5L, CD9?, CD98, CDA, CDC5L, CDK2 , CEACAM-8, CECR1, CENPF, CEP57, Carboxylesterase?1, CETP, Cezanne, CFHR 1, CFHR4, Complement Factor H-related 5, CFI, CFL1, Coagulation Factor VII, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, Collectin-12, CLPS, CLTA, CNN2, CNOT1, CO4A2, COG4?, COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Thrombospondin 5, Complement Factor B, Contactin-3, COPS8?, Corneodesmosin, Coronin 3, Cortactin, COTL1, Carboxypeptidase?E, CPEB3, Carboxypeptidase M, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin?A, Cyclophilin B, Cystatin D, Cystatin E/M, Cystatin?S, Cystatin SN, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 20, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DAN, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, DEP-1, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin?, DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, Ubiquitin-activating enzyme E1, ECHS1?, ECM-1, EEF1G?, EEF2, EFEMP2?, EFTUD2, EHD1?, EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1?, EMILIN1?, EMSY, EN2, Endorepellin, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, Aminopeptidase LRAP, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, Coagulation Factor XI, FABP5, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, F-box protein 38, FBPase 2, FBPase 1, FCGBP, FDPS?, FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FSH, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP?, GALNT2, Catenin gamma, GAPHD, GARNL1?, GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC?, GCSH?, GDA, GDF7, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, G6PD, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma , GSTO1, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, GPV, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLM1, GOLPH4?, GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GRP, GSTM1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, Growth Hormone, hHR23b, HIBADH?, HINT1, HIP1R?, Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha , hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G?, hnRNP L, hnRNP M1-M4?, hnRNP U?, Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRT, HPRG, HRSP12, HSC70, HSP47

Biotin-Labeled Lectin Mixture
?	Lectin Name	Sugar specificity
1	Concanavalin A	αMan, αGlc
2	Dolichos Biflorus Agglutinin	αGalNAc
3	Peanut Agglutinin	Galβ3GalNAc
4	Ulex Europaeus Lectin 1	αFuc
5	Wheat Germ Agglutinin	GlcNAc

Application Data/Notes

Typical Results

The following figure shows the RayBio? Human Glycosylation Antibody Array L3 probed with conditioned media sample. The images were captured using a Axon GenePix laser scanner. The strong signals in row 20 and the upper left and lower right corners of each array are Positive Controls, which can be used to identify the orientation and help normalize the results between arrays.

Glycosylation Antibody Array

Suggested Applications

Identify and profile the glycans in their samples
Determine whether their biomarker of interest has glycan moieties
Determine relative expression levels

Kit Components

Human Glycosylation Array L3 Glass Slide/s
Sample Diluent
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Biotin-labeled Lectin Mixture
Adhesive device sealer
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1 ml microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner

Protocol Outline

Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Human Glycosylation Array 500 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at –20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.

相關產品

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Anti-Xa21

Anti-HPT

Anti-CP4-EPSPS

文件

GAH-GCM-L3.pdf

文獻(2)

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses,Jia-An Cai, Yong-Zhen Zhang, En-Da Yu, Wei-Qun Ding, Zhao-Shen Li, Liang Zhong, Quan-Cai Cai,Tobacco Induced Diseases ,(IF:3.7) 作者單位：復旦大學附屬華山醫院
Species：	unknown
Sample Type：	unknown

View publications

Functionally enhanced cell spheroids for stem cell therapy: Role of TIMP1 in the survival and therapeutic effectiveness of stem cell spheroids,Zhong Qing Liang, Yong Bian, Jun Fei Gu, Gang Yin, Ruo Lan Sun, Yan Liang, Lin Lu Wan, Qi Hang Yin, Xu Wang, Jin Gao, Fan Zhao, De Cai Tang,PHYTOMEDICINE ,(IF:7.9) 作者單位：南京中醫藥大學
Species：	Human
Sample Type：	unknown

View publications

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Introduction

Product Features

Target Names

Biotin-Labeled Lectin Mixture

Application Data/Notes

Storage/Stability