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規格

規格	22樣本、50樣本、8樣本、22樣本(產品加服務)、8樣本(產品加服務)、50樣本(產品加服務)
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Rat
檢測靶標	IFN-gamma, TNF alpha, Decorin, IGF-1, IL-10, IL-13, IL-18, IL-2, IL-21, IL-4, LIF, VEGF-A, Cystatin C, EGFR, ErbB3, Erythropoietin, Galectin-3, GM-CSF, HGF, IL-6, Nope, PDGF-BB, RAGE, IL-5, ACE-2, Angiopoietin-2, IGFBP-1, IL-12 p70, Leptin, PDGF-AA, TIMP-1, TIMP-2, Beta-2 Microglobulin, Clusterin, CXCL16, EGF, VEGFR2, BID, GDNF, Activin A, Activin R2A, ADAM-9, BDNF, beta-NGF, BMP-7, Cathepsin L, CD14, CD38, CD47, CD59, CEACAM-1, CNTF, Contactin-1, Dkk-3, DLL1, E-Selectin, EphA3, EphA5, EphB3, EphB6, Fetuin B, FGF R4, FGF-12, FGF-21, FGF-BP, Flt-3 Ligand, Follistatin, FOLR1, Galectin-1, Gas 1, GDF-3, GFR alpha-2, Hemopexin, IL-17F, IL-22, IL-3, IL-31, IL-7, IL-9, Lactoferrin, Layilin, Legumain, Lumican, M-CSF R, MBL-2, MMP-2, MSP alpha, Neuropilin-2, Notch-1, P-Cadherin, P-Selectin, PDGF R alpha, Prolactin, RBP4, Syndecan-1, Syndecan-3, TGF beta R3, Transferrin, TREM-1, TRKB, VEGFR1, CRELD1, IFN alpha 5, MAG, Ameloblastin, Cadherin-4, Cathepsin E, CD164, CD28, CD34, CD5, CD6, CRELD2, Jagged 1, Neuroplastin, Notch-3, RGM-B, Semaphorin 4D, Sortilin, TrkA, CD5L, Desmocollin-2, ECM-1, Neuropilin-1, Notch-2, pIgR, Reg3A, Semaphorin 7A, SP-D, UCH-L1, Matrilin-4, ROBO1, SEMA3F, Noggin, Protocadherin-12, CDNF, CD83, Reg4, Cathepsin C, Prolactin R, IL-17RA, KLKB1, LIX, Olfactomedin-1, PGRPs, MESDC2, Plexin A1, Renin 1, IFN-gamma R2, Nephrin, CINC-2, CD1d1, CD300LG, CINC-3, CLEC4B2, COPZ1, Ephrin-A2, FCGR3A, FCGRT, GGT1, Neurexophilin-1, NPC2, Pentraxin-2, PGC, PLTP, PROCR, PSAP, PVRL1, Reg3B, Serpin A3N, Serpin E1, SGSH, Neurexophilin-3, STX7, beta, Heparin??Cofactor??II, IL-4R, Neurexin 1 alpha, Contactin-4 / BIG-2, Cathepsin B / Pro-Cathepsin B, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, MIP-1 alpha / CCL3, RANTES / CCL5, aFGF / FGF-1, IL-1 beta / IL-1F2, MIP-1 beta / CCL4, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Apolipoprotein H / ApoH, Carbonic Anhydrase XIV / CA14, VE-Cadherin / CDH5, Follistatin-like 1 / FSTL1, GFR alpha-3 / GDNF R alpha-3, IGSF8 / CD316, IL-11R alpha, IL-13R alpha 2, IL-15R alpha, IL-17RC, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b, IL-20R beta, IL-21R, IL-2R alpha, LRPAP / RAP, MDL-1 / CLEC5A, Neuroligin-1 / NLGN1, UNC5H1 / Netrin Receptor UNC5A, SCF / KITLG, Angiotensinogen / Serpin A8, Human Amyloid-like protein 1 / APLP-1, ASAH2 / Neutral Ceramidase, CD86 / B7-2, IGSF4B / SynCAM3 / CADM3, CD44 / Heparan Sulfate Proteoglycan, Contactin-3 / BIG-1, Coagulation Factor XIV / Protein C / F14, Carboxypeptidase A2 / CPA2, GDF-8 / Myostatin, GFR alpha-1 / GDNF R, JAM-C / CD323, Klotho beta / BKL, MCP-3 / MARC / CCL7, CD155 / PVR, SCGF / CLEC11a, Serpin D1 / Heparin??Cofactor??II, 4-1BB Ligand / TNFSF9, IL-1 alpha / IL-1F1, ALCAM / CD166, CD36 / SR-B3, CD40 / TNFRSF5, Endoglin / CD105, TROP1 / EpCAM, FCAR / CD89, GITR / TNFRSF18, IL-9R / CD129, JAM-A / CD321 / F11R, Lymphotoxin beta R / TNF RIII / TNFRSF3, Serpin F1 / PEDF, SIRP alpha / CD172a, IL-1ra / IL-1RN / IL-1 F3 / IRAP, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, TWEAK R / TNFRSF12, Fas / TNFRSF6 / Apo-1, 5'-Nucleotidase / CD73, Adiponectin / ACRP30, Aminopeptidase P2 / XPNPEP2, BAMBI / NMA, C1q R1 / CD93, C4.4A / LYPD3, Carboxypeptidase A1 / CPA1, Carboxypeptidase B1 / CPB1, CD48 / SLAMF2, CD80 / B7-1, Coagulation Factor III / Tissue Factor / Thromboplastin / F3, CRP / C-Reactive Protein, CTACK / CCL27, DNAM-1 / CD226, FABP4 / A-FABP, Fractalkine / CX3CL1, ICAM-1 / CD54, Iduronate 2-Sulfatase / IDS, IL-1Rrp2 / IL-1R-6, Kininogen / Kininostatin, Lipocalin-2 / NGAL, L-Selectin / CD62L, NAP-2 / PPBP / CXCL7, Osteopontin / SPP1, OX40 / TNFRSF4, Pref-1 / DLK-1 / FA1, RANK / TNFRSF11A, gp130 / sgp130, TIM-1 / KIM-1, 4-1BB / TNFRSF9 / CD137, Trypsin 3 / PRSS3, ADAMTS-1 / METH1, Fc gamma RIIB / CD32b, TCK-1 / CXCL7, BAFF / TNFSF13B, IL-2R gamma / Common gamma Chain, Heparan Sulfate 6-O-Sulfotransferase 3 / HS6ST3, Serpin F2 / Alpha-2-Antiplasmin, SIGNR1 / CD209b, IL-7R, Serpin A1c / Alpha-1-antitrypsin 1-3
靶標數	282

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), ACE-2, Activin A, Activin R2A, ADAM-9, ADAMTS-1 (METH1), Adiponectin (ACRP30), aFGF (FGF-1), ALCAM (CD166), Ameloblastin, Angiopoietin-2, Angiotensinogen (SERPINA8), ApoH, ASAH2 (Neutral Ceramidase), BAFF, BAMBI (NMA), BDNF, Beta-2 Microglobulin, beta-NGF, BID, BMP-7, BST1, C4.4A (LYPD3), Cadherin-4, Carbonic Anhydrase XIV (CA14), Carboxypeptidase A1 (CPA1), Carboxypeptidase B1 (CPB1), Cathepsin B (Pro-Cathepsin B), Cathepsin C, Cathepsin E, Cathepsin L, CD14, CD155 (PVR), CD164, CD1d1, CD226, CD28, CD300LG, CD34, CD36 (SR-B3), CD38, CD40 (TNFRSF5), CD44 (Heparan Sulfate Proteoglycan), CD47, CD48 (SLAMF2), CD5, CD59, CD5L, CD6, CD80 (B7-1), CD83, CD86 (B7-2), CD93, CDH5, CDNF, CEACAM-1, CINC-1 (CXCL1), CINC-2, CINC-3, CLEC4B2, CLEC5A, Clusterin, CNTF, Coagulation Factor III (Tissue Factor/Thromboplastin), Coagulation Factor XIV (Protein C), Contactin-1, Contactin-3 (BIG-1), Contactin-4 (BIG-2), COPZ1, CPA2, CRELD1, CRELD2, CRP (C-Reactive Protein), CSF1R, CTACK (CCL27), CXCL16, Cystatin C, Decorin, DKK-3, DLL1, DSC2, ECM-1, EGF, EGFR, Endoglin (CD105), Eotaxin-1 (CCL11), EphA3, EphA5, EphB3, EphB6, Ephrin-A2, ErbB3, Erythropoietin, E-Selectin, FABP4 (A-FABP), Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), FCAR (CD89), FCGR3A, FCGRT, FETUB, FGF R4, FGF-12, FGF-21, FGF-BP, Flt-3 Ligand, Follistatin, Follistatin-like 1, FOLR1, Fractalkine (CX3CL1), Galectin-1, Galectin-3, Gas 1, GDF-3, GDF-8 (Myostatin), GDNF, GFR alpha-1 (GDNF R), GFR alpha-2, GFR alpha-3, GGT1, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3), HGF, HPX, Human Amyloid-like protein 1 (APLP-1), ICAM-1 (CD54), Iduronate 2-Sulfatase (IDS), IFN alpha 5, IFN-gamma, IFN-gamma R2, IGF-1, IGFBP-1, IGSF4B (SynCAM3/CADM3), IGSF8 (CD316), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-11 R alpha, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15 R alpha, IL-17 RA, IL-17 RC, IL-17F, IL-18, IL-2, IL-2 R alpha, IL-2 R gamma (Common gamma Chain), IL-20 RB, IL-21, IL-21 R, IL-22, IL-3, IL-31, IL-4, IL-4R, IL-5, IL-6, IL-7, IL-7 R, IL-9, IL-9 R (CD129), Jagged 1, JAM-A (CD321/F11R), JAM-C (CD323), KDR, Kininogen (Kininostatin), KLKB1, Klotho beta, Lactoferrin, Layilin, Legumain, Leptin, LIF, Lipocalin-2 (NGAL), LIX, LRPAP, L-Selectin (CD62L), Lumican, Lymphotoxin beta R (TNF RIII), MAG, Matrilin-4, MBL-2, MCP-1 (CCL2), MCP-3 (MARC/CCL7), MESDC2, MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MMP-2, MSP alpha/beta, Nephrin, Neurexin 1 alpha, Neurexophilin-1, Neurexophilin-3, Neuroligin-1 (NLGN1), Neuropilin-1, Neuropilin-2, Neuroplastin, Noggin, Nope, Notch-1, Notch-2, Notch-3, NPC2, NT5E, Olfactomedin-1, Osteopontin (SPP1), OX40 (TNFRSF4), P-Cadherin, PDGF R alpha, PDGF-AA, PDGF-BB, Pentraxin-2, PGC, PGRPs, pIgR, Plexin A1, PLTP, Pref-1 (DLK-1/FA1), PROCR, Prolactin, Prolactin R, Protocadherin-12, PSAP, P-Selectin, PVRL1, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Reg3A, Reg3B, Reg4, Renin 1, RGM-B, ROBO1, SCF, SCGF (CLEC11a), SEMA3F, Semaphorin 4D, Semaphorin 7A, SERPINA1c, SERPINA3N, SERPIND1 (Heparin?Cofactor?II), SERPINE1, SERPINF1 (PEDF), SERPINF2 (Alpha-2-Antiplasmin), SGSH, SIGNR1 (CD209b), SIRP alpha, Sortilin, SP-D, STX7, Syndecan-1, Syndecan-3, TCK-1 (CXCL7), TGF beta R3, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, Transferrin, TREM-1, TrkA, TRKB, TROP1 (EpCAM), Trypsin 3 (PRSS3), TWEAK R (TNFRSF12), UCH-L1, UNC5H1 (Netrin Receptor UNC5A), VEGF-A, VEGFR1, XPNPEP2

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Rat Cytokine Array Q282 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktails
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAR-CAA-282.pdf

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