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規格

規格	22樣本(產品加服務)、8樣本(產品加服務)、8樣本、50樣本(產品加服務)、22樣本、50樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	Angiopoietin-1, Resistin, AgRP, PAI-1, PDGF-AB, TGF beta 1, Angiogenin, Angiostatin, TGF beta 2, Activin A, Cathepsin S, Cripto-1, DAN, DKK-1, E-Cadherin, Follistatin, Galectin-7, IL-17B, IL-23, Nr-CAM, TREM-1, VEGF-C, VEGFR1, LAP/TGF beta 1, Fas Ligand / TNFSF6, IL-13R alpha 2, IL-2R alpha, Tie-2 / TEK, ICAM-2 / CD102, IL-13R alpha 1, IL-1R-4 / ST2 / IL-33R, Siglec-5 / CD170 / Siglec-F, CD40 / TNFRSF5, TROP1 / EpCAM, Fc gamma RIIB/C / CD32B/C, IL-2R beta / CD122, SDF-1 beta / CXCL12 beta, gp130 / sgp130, Sonic Hedgehog N-Terminal / Shh-N, Siglec-5 / CD170, Thrombopoietin / TPO, TRAIL R4 / TNFRSF10D
靶標數	40

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747 ELISA Kit: RayBio Human Angiostatin ELISA
Cathepsin S Gene Symbol: CTSS Gene ID: 1520 Accession #: P25774 ELISA Kit: RayBio Human Cathepsin S ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	Cripto-1 Gene Symbol: TDGF1 Gene ID: 6997 Accession #: P13385	DAN Gene Symbol: PARN Gene ID: 5073 Accession #: O95453 ELISA Kit: RayBio Human DAN ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA
E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	Fc gamma RIIB/C (CD32B/C) Gene Symbol: FCGR2B Gene ID: 2213 Accession #: P31994 ELISA Kit: RayBio Human FcgRIIBC ELISA	Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	Galectin-7 Gene Symbol: LGALS7 Gene ID: 3963 Accession #: P47929 ELISA Kit: RayBio Human Galectin-7 ELISA
gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	ICAM-2 (CD102) Gene Symbol: ICAM2 Gene ID: 3384 Accession #: P13598 ELISA Kit: RayBio Human ICAM-2 ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-13 R alpha 2 Gene Symbol: IL13RA2 Gene ID: 3598 Accession #: Q14627 ELISA Kit: RayBio Human IL-13 Ra2 ELISA	IL-13 R1
IL-17B Gene Symbol: IL17B Gene ID: 27190 Accession #: Q9UHF5 ELISA Kit: RayBio Human IL-17B ELISA	IL-2 R alpha Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha?ELISA	IL-2 R beta (CD122) Gene Symbol: IL2RB Gene ID: 3560 Accession #: P14784 ELISA Kit: RayBio Human IL-2 R beta?ELISA	IL-23 Gene Symbol: IL23A Gene ID: 51561 Accession #: Q9NPF7 ELISA Kit: RayBio Human IL-23 ELISA	LAP/TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137
NrCAM Gene Symbol: NrCAM Gene ID: 4897 Accession #: Q92823 ELISA Kit: RayBio Human NRCAM ELISA	PAI-1 Gene Symbol: SERPINE1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA	PDGF-AB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-AB ELISA	Resistin Gene Symbol: RETN Gene ID: 56729 Accession #: Q9HD89 ELISA Kit: RayBio Human Resistin ELISA	SDF-1 beta (CXCL12 beta) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 beta ELISA
Siglec-5 (CD170) Gene Symbol: SIGLEC5 Gene ID: 8778 Accession #: O15389 ELISA Kit: RayBio Human Siglec-5 ELISA	Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: 6469 Accession #: Q15465 ELISA Kit: RayBio Human ShhN ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Tie-2 ELISA
TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	TREM-1 Gene Symbol: TREM1 Gene ID: 54210 Accession #: Q9NP99 ELISA Kit: RayBio Human TREM-1 ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1?ELISA	VEGF-C Gene Symbol: VEGFC Gene ID: 7424 Accession #: P49767 ELISA Kit: RayBio Human VEGF-C ELISA	VEGFR1 Gene Symbol: Flt1 Gene ID: 2321 Accession #: P17948 ELISA Kit: RayBio Human VEGF R1 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q4 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAH-CYT-4.pdf

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