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規(guī)格

規(guī)格	50樣本、8樣本、22樣本、8樣本(產(chǎn)品加服務(wù))、22樣本(產(chǎn)品加服務(wù))、50樣本(產(chǎn)品加服務(wù))
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標(biāo)	bax, Caspase-3, C-Peptide, LCK, S100 B, ZAP70, FAM3A, HAPLN4, IRF-4, PLA2G4A, RalB, S100 A2, SH2B1, ST8SIA2, VAP-B, Dystroglycan, HMGB3, SOD3, AHCY, GCNT1, Protocadherin-17, PI3-Kinase p55-gamma, hCG intact, BNIP3, Cathepsin F, CD177, Siglec-3 / CD33, Testican 1 / SPOCK1, TRP14 / Thioredoxin-like 5, Activin C / INHBC, Alpha-Galactosidase A / GLA, Klotho beta / BKL, LILRA5 / ILT-11 / CD85f, APE1 / APEX1, CD96 Isoform 2 / Tactile, CLEC4D / CLECSF8 / CLEC-6, DCBLD2 / ESDN, IkB-epsilon / NF-kappa-BIE, KIR3DL1 / CD158e, METTL11A / NRMT
靶標(biāo)數(shù)	40

詳細(xì)描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Activin C (INHBC) Gene Symbol: INHBC Gene ID: 3626 Accession #: P55103 ELISA Kit: RayBio Human Activin C/INHBC ELISA	AHCY Gene Symbol: INHBC Gene ID: 3626 Accession #: P55103 ELISA Kit: RayBio Human Activin C/INHBC ELISA	Alpha-Galactosidase A (GLA) Gene Symbol: AHCY SAHH Gene ID: 191 Accession #: P23526 ELISA Kit: RayBio Custom Human AHCY ELISA	APE1 (APEX1) Gene ID: 2717 Accession #: P06280 ELISA Kit: RayBio Human Alpha-Galactosidase A/GLA ELISA	bax Gene ID: 328 Accession #: P27695 ELISA Kit: RayBio Human APE1/APEX1 ELISA
BNIP3 Gene Symbol: BAX BCL2L4 Gene ID: 581 Accession #: Q07812 ELISA Kit: RayBio	Caspase-3 Gene Symbol: BNIP3 NIP3 Gene ID: 664 Accession #: Q12983 ELISA Kit: RayBio Custom Human BNIP3 ELISA	Cathepsin F Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio	CD177 Gene Symbol: CTSF Gene ID: 8722 Accession #: Q9UBX1 ELISA Kit: RayBio Custom Human Cathepsin F ELISA	CD96 Isoform 2 (Tactile) Gene Symbol: CD177 NB1 PRV1 UNQ595/PRO1181 Gene ID: 57126 Accession #: Q8N6Q3 ELISA Kit: RayBio Human CD177 ELISA
CLEC4D (CLECSF8/CLEC-6) Gene Symbol: CD96 Accession #: P40200-2 ELISA Kit: RayBio Custom Human CD96 Isoform 2/Tactile ELISA	C-Peptide Gene Symbol: CLEC4D CLECSF8 MCL Gene ID: 338339 Accession #: Q8WXI8 ELISA Kit: RayBio Custom Human CLEC4D/CLECSF8/CLEC-6 ELISA	DCBLD2 (ESDN) Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human C-Peptide ELISA	Dystroglycan Gene Symbol: CLCP1 DCBLD2 ESDN Gene ID: 131566 Accession #: Q96PD2 ELISA Kit: RayBio Human DCBLD2/ESDN ELISA	FAM3A Gene Symbol: DAG1 Gene ID: 1605 Accession #: Q14118 ELISA Kit: RayBio Custom Human Dystroglycan ELISA
GCNT1 Gene Symbol: 2-19 2.19 FAM3A Gene ID: 60343 Accession #: P98173 ELISA Kit: RayBio Custom Human FAM3A ELISA	HAPLN4 Gene Symbol: GCNT1 NACGT2 Gene ID: 2650 Accession #: Q02742 ELISA Kit: RayBio Custom Human GCNT1 ELISA	hCG intact Gene Symbol: BRAL2 HAPLN4 KIAA1926 Gene ID: 404037 Accession #: Q86UW8 ELISA Kit: RayBio Custom Human HAPLN4 ELISA	HMGB3 Gene Symbol: HMG2A HMG4 HMGB3 Gene ID: 3149 Accession #: O15347 ELISA Kit: RayBio Custom Human HMGB3 ELISA	IkB-epsilon (NF-kappa-BIE) Gene Symbol: IKBE NFKBIE Gene ID: 4794 Accession #: O00221 ELISA Kit: RayBio Custom Human IkB-epsilon/NF-kappa-BIE ELISA
IRF-4 Gene Symbol: IRF4 MUM1 Gene ID: 3662 Accession #: Q15306 ELISA Kit: RayBio Custom Human IRF-4 ELISA	KIR3DL1 (CD158e) Gene Symbol: CD158E KIR3DL1 NKAT3 NKB1 Gene ID: 3811 Accession #: P43629 ELISA Kit: RayBio Custom Human KIR3DL1/CD158e ELISA	Klotho beta Gene Symbol: KLB Gene ID: 152831 Accession #: Q86Z14 ELISA Kit: RayBio Custom Human Klotho beta/BKL ELISA	LCK Gene Symbol: LCK Gene ID: 3932 Accession #: P06239 ELISA Kit: RayBio Custom Human LCK ELISA	LILRA5 (ILT-11/CD85f) Gene Symbol: ILT11 LILRA5 LILRB7 LIR9 Gene ID: 353514 Accession #: A6NI73 ELISA Kit: RayBio Custom Human LILRA5/ILT-11/CD85f ELISA
METTL11A (NRMT) Gene Symbol: AD-003 C9ORF32 METTL11A NRMT NRMT1 NTMT1 Gene ID: 28989 Accession #: Q9BV86 ELISA Kit: RayBio Custom Human METTL11A/NRMT ELISA	PI3-Kinase p55-gamma Gene Symbol: PIK3R3 Gene ID: 8503 Accession #: Q92569 ELISA Kit: RayBio Custom Human PI3-Kinase p55-gamma ELISA	PLA2G4A Gene Symbol: CPLA2 PLA2G4 PLA2G4A Gene ID: 5321 Accession #: P47712 ELISA Kit: RayBio Custom Human PLA2G4A ELISA	Protocadherin-17 Gene Symbol: PCDH17 PCDH68 PCH68 Gene ID: 27253 Accession #: O14917 ELISA Kit: RayBio Custom Human Protocadherin-17 ELISA	RalB Gene Symbol: RALB Gene ID: 5899 Accession #: P11234 ELISA Kit: RayBio Custom Human RalB ELISA
S100 A2 Gene Symbol: S100A2 S100L Gene ID: 6273 Accession #: P29034 ELISA Kit: RayBio Human S100 A2 ELISA	S100 B Gene Symbol: S100B Gene ID: 6285 Accession #: P04271 ELISA Kit: RayBio	SH2B1 Gene Symbol: KIAA1299 SH2B SH2B1 Gene ID: 25970 Accession #: Q9NRF2 ELISA Kit: RayBio Custom Human SH2B1 ELISA	Siglec-3 Gene Symbol: CD33 SIGLEC3 Gene ID: 945 Accession #: P20138 ELISA Kit: RayBio Human Siglec-3 ELISA	SOD3 Gene ID: 6649 Accession #: P08294 ELISA Kit: RayBio Human SOD3 ELISA
ST8SIA2 Gene Symbol: SIAT8B ST8SIA2 STX Gene ID: 8128 Accession #: Q92186 ELISA Kit: RayBio Human ST8SIA2 ELISA	Testican 1 (SPOCK1) Gene Symbol: SPOCK1 SPOCK TIC1 TICN1 Gene ID: 6695 Accession #: Q08629 ELISA Kit: RayBio Custom Human Testican 1/SPOCK1 ELISA	TRP14 (Thioredoxin-like 5) Gene Symbol: TXNDC17 TXNL5 Gene ID: 84817 Accession #: Q9BRA2 ELISA Kit: RayBio Custom Human TRP14/Thioredoxin-like 5 ELISA	VAP-B Gene Symbol: VAPB UNQ484/PRO983 Gene ID: 9217 Accession #: O95292 ELISA Kit: RayBio Custom Human VAP-B ELISA	ZAP70 Gene Symbol: SRK ZAP70 Gene ID: 7535 Accession #: P43403 ELISA Kit: RayBio Custom Human ZAP70 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q28 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAH-CYT-28.pdf

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