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規(guī)格

規(guī)格	8樣本、22樣本、22樣本(產(chǎn)品加服務(wù))、8樣本(產(chǎn)品加服務(wù))、50樣本(產(chǎn)品加服務(wù))、50樣本
樣本類(lèi)型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測(cè)方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測(cè)靶標(biāo)	GSK-3 beta, FGF R3, Mesothelin, HNMT, ACP6, BAI3, Caspase-1, Cyclin E1, FUT11, IRF-2, JunB, Latexin, NFKB1, PTP-MEG2, Annexin A4, CD160, CDK5 Activator 1, Complexin-1, Doc2-alpha, Galectin-10, GMF-beta, ISLR-2, Oligo3, PAG1, Pro-EGF, LMW-PTP / ACP-1, ASAM / ACAM, DC-SIGN / CD209, BAK / Bcl2-L-7, Brachyury / Protein T, BTLA / CD272, DLEC / CLEC4C / BDCA-2, ER alpha / NR3A1, FKBPR38 / PPIase FKBP8, FXR / Bile Acid Receptor, Glucokinase / GCK, GLB1 / Beta-galactosidase, IKK-gamma / NEMO / FIP-3, p130Cas / BCAR1 / CAS, PGCD6 / ALG-2
靶標(biāo)數(shù)	40

詳細(xì)描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
ACP6 Gene ID: 51205 Accession #: Q9NPH0 ELISA Kit: RayBio Human ACP6 ELISA	Annexin A4 Gene ID: 51205 Accession #: Q9NPH0 ELISA Kit: RayBio Human ACP6 ELISA	ASAM (ACAM) Gene Symbol: ANX4 ANXA4 Gene ID: 307 Accession #: P09525 ELISA Kit: RayBio Custom Human Annexin A4 ELISA	BAI3 Gene Symbol: ACAM ASAM ASP5 CLMP Gene ID: 71566 Accession #: Q8R373 ELISA Kit: RayBio Custom Human ASAM/ACAM ELISA	BAK (Bcl2-L-7) Gene Symbol: ADGRB3 BAI3 KIAA0550 Gene ID: 577 Accession #: O60242 ELISA Kit: RayBio Custom Human BAI3 ELISA
Brachyury (Protein T) Gene Symbol: BAK BAK1 BCL2L7 CDN1 Gene ID: 578 Accession #: Q16611 ELISA Kit: RayBio Custom Human BAK/Bcl2-L-7 ELISA	BTLA (CD272) Gene Symbol: T Gene ID: 6862 Accession #: O15178 ELISA Kit: RayBio	Caspase-1 Gene Symbol: BTLA Gene ID: 151888 Accession #: Q7Z6A9 ELISA Kit: RayBio Custom Human BTLA/CD272 ELISA	CD160 Gene Symbol: ELH-CASP1 Gene ID: 834 Accession #: P29466 ELISA Kit: RayBio Human Caspase-1 ELISA	CDK5 Activator 1 Gene Symbol: BY55 CD160 Gene ID: 11126 Accession #: O95971 ELISA Kit: RayBio Custom Human CD160 ELISA
Complexin-1 Gene Symbol: CDK5R CDK5R1 NCK5A Gene ID: 8851 Accession #: Q15078 ELISA Kit: RayBio Custom Human CDK5 Activator 1 ELISA	Cyclin E1 Gene Symbol: CPLX1 Gene ID: 10815 Accession #: O14810 ELISA Kit: RayBio Custom Human Complexin-1 ELISA	DC-SIGN (CD209) Gene Symbol: CCNE CCNE1 Gene ID: 898 Accession #: P24864 ELISA Kit: RayBio Custom Human Cyclin E1 ELISA	DLEC (CLEC4C/BDCA-2) Gene Symbol: CD209 CLEC4L Gene ID: 30835 Accession #: Q9NNX6 ELISA Kit: RayBio Custom Human DC-SIGN/CD209 ELISA	Doc2-alpha Gene Symbol: BDCA2 CLEC4C CLECSF11 CLECSF7 DLEC HECL UNQ9361/PRO34150 Gene ID: 170482 Accession #: Q8WTT0 ELISA Kit: RayBio Human DLEC/CLEC4C/BDCA-2 ELISA
ER alpha (NR3A1) Gene Symbol: DOC2A Gene ID: 8448 Accession #: Q14183 ELISA Kit: RayBio Custom Human Doc2-alpha ELISA	FGF R3 Gene Symbol: ESR ESR1 NR3A1 Gene ID: 2099 Accession #: P03372 ELISA Kit: RayBio Custom Human ER alpha/NR3A1 ELISA	FKBPR38 (PPIase FKBP8) Gene Symbol: FGFR3 JTK4 Gene ID: 2261 Accession #: P22607 ELISA Kit: RayBio Custom Human FGF R3 ELISA	FUT11 Gene Symbol: FUT11 Gene ID: 170384 Accession #: Q495W5 ELISA Kit: RayBio Custom Human FUT11 ELISA	FXR (Bile Acid Receptor) Gene Symbol: BAR FXR HRR1 NR1H4 RIP14 Gene ID: 9971 Accession #: Q96RI1 ELISA Kit: RayBio Custom Human FXR/Bile Acid Receptor ELISA
Galectin-10 Gene ID: 1178 Accession #: Q05315 ELISA Kit: RayBio Human Galectin-10 ELISA	GLB1 (Beta-galactosidase) Gene Symbol: ELNR1 GLB1 Gene ID: 2720 Accession #: P16278 ELISA Kit: RayBio Custom Human GLB1/Beta-galactosidase ELISA	Glucokinase (GCK) Gene Symbol: GCK Gene ID: 2645 Accession #: P35557 ELISA Kit: RayBio Custom Human Glucokinase/GCK ELISA	GMF-beta Gene Symbol: GMFB Gene ID: 2764 Accession #: P60983 ELISA Kit: RayBio Custom Human GMF-beta ELISA	GSK-3 beta Gene Symbol: GSK3B Gene ID: 2932 Accession #: P49841 ELISA Kit: RayBio Custom Human GSK-3 beta ELISA
HNMT Gene Symbol: HNMT Gene ID: 3176 Accession #: P50135 ELISA Kit: RayBio Custom Human HNMT ELISA	IKK-gamma (NEMO/FIP-3) Gene Symbol: FIP3 IKBKG NEMO Gene ID: 8517 Accession #: Q9Y6K9 ELISA Kit: RayBio Custom Human IKK-gamma/NEMO/FIP-3 ELISA	IRF-2 Gene Symbol: IRF2 Gene ID: 3660 Accession #: P14316 ELISA Kit: RayBio Custom Human IRF-2 ELISA	ISLR-2 Gene Symbol: ISLR2 KIAA1465 LINX UNQ1885/PRO4329 Gene ID: 57611 Accession #: Q6UXK2 ELISA Kit: RayBio Custom Human ISLR-2 ELISA	JunB Gene Symbol: JUNB Gene ID: 3726 Accession #: P17275 ELISA Kit: RayBio Custom Human JunB ELISA
Latexin Gene ID: 56925 Accession #: Q9BS40 ELISA Kit: RayBio Human Latexin ELISA	LMW-PTP (ACP-1) Gene Symbol: ACP1 Accession #: P24666-2 ELISA Kit: RayBio Custom Human LMW-PTP/ACP-1 ELISA	Mesothelin Gene Symbol: MSLN MPF Gene ID: 10232 Accession #: Q13421 ELISA Kit: RayBio Human Mesothelin ELISA	NFKB1 Gene Symbol: NFKB1 Accession #: P19838-2 ELISA Kit: RayBio Custom Human NFKB1 ELISA	Oligo3 Gene Symbol: BHLHB7 BHLHE20 OLIG3 Gene ID: 167826 Accession #: Q7RTU3 ELISA Kit: RayBio Custom Human Oligo3 ELISA
p130Cas (BCAR1/CAS) Gene Symbol: BCAR1 CAS CASS1 CRKAS Gene ID: 9564 Accession #: P56945 ELISA Kit: RayBio Custom Human p130Cas/BCAR1/CAS ELISA	PAG1 Gene Symbol: CBP PAG PAG1 Gene ID: 55824 Accession #: Q9NWQ8 ELISA Kit: RayBio Custom Human PAG1 ELISA	PGCD6 (ALG-2) Gene Symbol: ALG2 PDCD6 Gene ID: 10016 Accession #: O75340 ELISA Kit: RayBio Custom Human PGCD6/ALG-2 ELISA	Pro-EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Custom Human Pro-EGF ELISA	PTP-MEG2 Gene Symbol: PTPN9 Gene ID: 5780 Accession #: P43378 ELISA Kit: RayBio Custom Human PTP-MEG2 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q25 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAH-CYT-25.pdf

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