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規格

規格	8樣本、22樣本(產品加服務)、22樣本、50樣本、8樣本(產品加服務)、50樣本(產品加服務)
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	PLGF, EphB2, EphB3, Galanin, PON1, Contactin-5, Fgr, GALNT10, HS3ST1, HS3ST3B1, LOXL2, MAP1D, Matrilin-2, MEF2C, SorCS2, ST3GAL1, ST8SIA1, Cathepsin H, FKBP51, FUCA1, Bcl2-L-3, C1qTNF9, CA5A, Glyoxalase 2, Lin-28A, LRRTM4, MCEMP1, METAP2, Neurocan, SALM4, Semaphorin 6C, Mcl-1 / Bcl2L3, Draxin / Neucrin, CANT1 / SCAN-1, CTRC / Chymotrypsin-C / Caldecrin, FABP8 / M-FABP, Gastrokine-1 / GKN1, MDGA2 / MAMDC1, Nogo-A / Reticulon 4A, PCK1 / PEPCK-C, Tsukushi / TSK
靶標數	40

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
C1qTNF9 Gene Symbol: C1QTNF9 C1QTNF9A UNQ6503/PRO21380 Gene ID: 338872 Accession #: P0C862 ELISA Kit: RayBio Custom Human C1qTNF9 ELISA	CA5A Gene Symbol: CA5A CA5 Gene ID: 763 Accession #: P35218 ELISA Kit: RayBio Custom Human CA5A ELISA	CANT1 (SCAN-1) Gene Symbol: CANT1 SHAPY Gene ID: 124583 Accession #: Q8WVQ1 ELISA Kit: RayBio Custom Human CANT1/SCAN-1 ELISA	Cathepsin H Gene Symbol: CTSH CPSB Gene ID: 1512 Accession #: P09668#PRO_0000026209	Contactin-5 Gene Symbol: CNTN5 Gene ID: 53942 Accession #: O94779 ELISA Kit: RayBio Custom Human Contactin-5 ELISA
CTRC (Chymotrypsin-C/Caldecrin) Gene Symbol: CTRC CLCR Gene ID: 11330 Accession #: Q99895 ELISA Kit: RayBio Custom Human CTRC/Chymotrypsin-C/Caldecrin ELISA	Draxin (Neucrin) Gene Symbol: DRAXIN C1orf187 PSEC0258 UNQ3119/PRO10268 Gene ID: 374946 Accession #: Q8NBI3 ELISA Kit: RayBio Custom Human Draxin/Neucrin ELISA	EphB2 Gene Symbol: EPHB2 DRT EPHT3 EPTH3 ERK HEK5 TYRO5 Gene ID: 2048 Accession #: P29323 ELISA Kit: RayBio Custom Human EphB2 ELISA	EphB3 Gene Symbol: EPHB3 ETK2 HEK2 TYRO6 Gene ID: 2049 Accession #: P54753 ELISA Kit: RayBio Custom Human EphB3 ELISA	FABP8 (M-FABP) Gene Symbol: PMP2 Gene ID: 5375 Accession #: P02689 ELISA Kit: RayBio Custom Human FABP8/M-FABP ELISA
Fgr Gene Symbol: FGR SRC2 Gene ID: 2268 Accession #: P09769 ELISA Kit: RayBio Custom Human Fgr ELISA	FKBP51 Gene Symbol: FKBP5 AIG6 FKBP51 Gene ID: 2289 Accession #: Q13451 ELISA Kit: RayBio Custom Human FKBP51 ELISA	FUCA1 Gene Symbol: FUCA1 Nbla10230 Gene ID: 2517 Accession #: P04066 ELISA Kit: RayBio Custom Human FUCA1 ELISA	Galanin Gene Symbol: GAL GAL1 GALN GLNN Gene ID: 51083 Accession #: P22466 ELISA Kit: RayBio Custom Human Galanin ELISA	GALNT10 Gene Symbol: GALNT10 Gene ID: 55568 Accession #: Q86SR1 ELISA Kit: RayBio Custom Human GALNT10 ELISA
Gastrokine-1 (GKN1) Gene Symbol: GKN1 AMP18 CA11 UNQ489/PRO1005 Gene ID: 56287 Accession #: Q9NS71 ELISA Kit: RayBio Custom Human Gastrokine-1/GKN1 ELISA	Glyoxalase II Gene Symbol: HAGH GLO2 HAGH1 Gene ID: 3029 Accession #: Q16775 ELISA Kit: RayBio Custom Human Glyoxalase II ELISA	HS3ST1 Gene Symbol: HS3ST1 3OST 3OST1 Gene ID: 9957 Accession #: O14792 ELISA Kit: RayBio Custom Human HS3ST1 ELISA	HS3ST3B1 Gene Symbol: HS3ST3B1 3OST3B1 HS3ST3B Gene ID: 9953 Accession #: Q9Y662 ELISA Kit: RayBio Custom Human HS3ST3B1 ELISA	Lin-28A Gene Symbol: LIN28A CSDD1 LIN28 ZCCHC1 Gene ID: 79727 Accession #: Q9H9Z2 ELISA Kit: RayBio Custom Human Lin-28A ELISA
LOXL2 Gene Symbol: LOXL2 Gene ID: 4017 Accession #: Q9Y4K0 ELISA Kit: RayBio Custom Human LOXL2 ELISA	LRRTM4 Gene Symbol: LRRTM4 UNQ3075/PRO9907 Gene ID: 80059 Accession #: Q86VH4 ELISA Kit: RayBio Custom Human LRRTM4 ELISA	MAP1D Gene Symbol: METAP1D MAP1D Gene ID: 254042 Accession #: Q6UB28 ELISA Kit: RayBio Custom Human MAP1D ELISA	Matrilin-2 Gene Symbol: MATN2 UNQ193/PRO219 Gene ID: 4147 Accession #: O00339 ELISA Kit: RayBio Custom Human Matrilin-2 ELISA	MCEMP1 Gene Symbol: MCEMP1 C19orf59 Gene ID: 199675 Accession #: Q8IX19 ELISA Kit: RayBio Custom Human MCEMP1 ELISA
Mcl-1 (Bcl2L3) Gene Symbol: MCL1 BCL2L3 Gene ID: 4170 Accession #: Q07820 ELISA Kit: RayBio Custom Human Mcl-1/Bcl2L3 ELISA	MDGA2 (MAMDC1) Gene Symbol: MDGA2 MAMDC1 UNQ8188/PRO23197 Gene ID: 161357 Accession #: Q7Z553 ELISA Kit: RayBio Custom Human MDGA2/MAMDC1 ELISA	MEF2C Gene Symbol: MEF2C Gene ID: 4208 Accession #: Q06413 ELISA Kit: RayBio Custom Human MEF2C ELISA	METAP2 Gene Symbol: METAP2 MNPEP P67EIF2 Gene ID: 10988 Accession #: P50579 ELISA Kit: RayBio Custom Human METAP2 ELISA	Neurocan Gene Symbol: NCAN CSPG3 NEUR Gene ID: 1463 Accession #: O14594 ELISA Kit: RayBio Custom Human Neurocan ELISA
Nogo-A (Reticulon 4A) Gene Symbol: Rtn4 Nogo Gene ID: 57142 Accession #: Q9NQC3 ELISA Kit: RayBio Custom Human Nogo-A/Reticulon-4 ELISA	PCK1 (PEPCK-C) Gene Symbol: PCK1 PEPCK1 Gene ID: 5105 Accession #: P35558 ELISA Kit: RayBio Custom Human PCK1/PEPCK-C ELISA	PlGF-2 Gene Symbol: PGF PGFL PLGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	PON1 Gene Symbol: PON1 PON Gene ID: 5444 Accession #: P27169 ELISA Kit: RayBio Custom Human PON1 ELISA	SALM4 Gene Symbol: LRFN3 SALM4 UNQ5865/PRO34192 Gene ID: 79414 Accession #: Q9BTN0 ELISA Kit: RayBio Custom Human SALM4 ELISA
Semaphorin 6C Gene Symbol: SEMA6C KIAA1869 SEMAY Gene ID: 10500 Accession #: Q9H3T2 ELISA Kit: RayBio Custom Human Semaphorin 6C ELISA	SorCS2 Gene Symbol: SORCS2 KIAA1329 Gene ID: 57537 Accession #: Q96PQ0 ELISA Kit: RayBio Custom Human SorCS2 ELISA	ST3GAL1 Gene Symbol: ST3GAL1 SIAT4 SIAT4A Gene ID: 6482 Accession #: Q11201 ELISA Kit: RayBio Custom Human ST3GAL1 ELISA	ST8SIA1 Gene Symbol: ST8SIA1 SIAT8 SIAT8A Gene ID: 6489 Accession #: Q92185 ELISA Kit: RayBio Custom Human ST8SIA1 ELISA	Tsukushi (TSK) Gene Symbol: TSKU E2IG4 LRRC54 TSK UNQ850/PRO1788 Gene ID: 25987 Accession #: Q8WUA8 ELISA Kit: RayBio Custom Human Tsukushi/TSK ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q22 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAH-CYT-22.pdf

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