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規格

規格	8樣本、8樣本(產品加服務)、22樣本(產品加服務)、50樣本(產品加服務)、22樣本、50樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	FABP1, L-FAP1, p53, BIRC4, IAP-3, XIAP, CD23, Fc epsilon RII, HAI-1, IL-27, LIF R alpha, Lipocalin-1, LRP-6, Mesothelin, MFRP, Neuropilin-2, Neurturin, PDGF-C, Progranulin, Ret, ROBO4, TCCR, TLR1, TLR3, WSX-1, HIF-1 alpha, TREM-2, Nidogen-2, Semaphorin 6B, ADAM-23, TRKC, IL-27R alpha, Insulin R / CD220, GDF-8 / Myostatin, Lymphotoxin beta R / TNF RIII / TNFRSF3, Serpin F1 / PEDF, ACE / CD143, Activin R1B / ALK-4, Cardiotrophin-1 / CT-1, NRG1-alpha / HRG1-alpha, Olfactomedin-2 / Noelin-2, SREC-I / SCARF1, SREC-II / SCARF2, Tripeptidyl-peptidase I / TPP1, TROY / TNFRSF19, PD-ECGF / Thymidine Phosphorylase
靶標數	40

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
ACE (CD143) Gene Symbol: ACE DCP DCP1 Gene ID: 1636 Accession #: P12821 ELISA Kit: RayBio Human ACE ELISA	Activin R1B (ALK-4) Gene Symbol: ACVR1B ACVRLK4 ALK4 Gene ID: 91 Accession #: P36896 ELISA Kit: RayBio Human Activin R1B ELISA	ADAM23 Gene Symbol: ADAM23 MDC3 Gene ID: 8745 Accession #: O75077 ELISA Kit: RayBio Human ADAM23 ELISA	Artemin Gene Symbol: ARTN EVN Gene ID: 9048 Accession #: Q5T4W7 ELISA Kit: RayBio Human Artemin ELISA	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: 1489 Accession #: Q16619 ELISA Kit: RayBio Human CT-1 ELISA
Cathepsin V (Cathepsin L2) Gene Symbol: CTSV CATL2 CTSL2 CTSU UNQ268/PRO305 Gene ID: 1515 Accession #: O60911 ELISA Kit: RayBio Human Cathepsin V ELISA	FABP1 Gene Symbol: FABP1 FABPL Gene ID: 2168 Accession #: P07148	FGF-20 Gene Symbol: FGF20 Gene ID: 26281 Accession #: Q9NP95 ELISA Kit: RayBio Human FGF-20 ELISA	GDF-8 (Myostatin) Gene Symbol: MSTN GDF8 Gene ID: 2660 Accession #: O14793 ELISA Kit: RayBio Human GDF-8 ELISA	HAI-1 Gene Symbol: SPINT1 HAI1 UNQ223/PRO256 Gene ID: 6692 Accession #: O43278 ELISA Kit: RayBio Human HAI-1 ELISA
IL-27 R alpha Gene Symbol: IL27RA CRL1 TCCR WSX1 UNQ296/PRO336 Gene ID: 9466 Accession #: Q6UWB1 ELISA Kit: RayBio Human IL-27 R alpha ELISA	Insulin R (CD220) Gene Symbol: INSR Gene ID: 3643 Accession #: P06213	Kallikrein 7 Gene Symbol: KLK7 PRSS6 SCCE Gene ID: 5650 Accession #: P49862 ELISA Kit: RayBio Human Kallikrein 7 ELISA	LIF R alpha Gene Symbol: LIFR Gene ID: 3977 Accession #: P42702 ELISA Kit: RayBio Human LIF R alpha ELISA	Lipocalin-1 Gene Symbol: LCN1 VEGP Gene ID: 3933 Accession #: P31025 ELISA Kit: RayBio Human Lipocalin-1 ELISA
Lymphotoxin beta R (TNF RIII) Gene Symbol: LTBR D12S370 TNFCR TNFR3 TNFRSF3 Gene ID: 4055 Accession #: P36941 ELISA Kit: RayBio Human Lymphotoxin beta R ELISA	Mesothelin Gene Symbol: MSLN MPF Gene ID: 10232 Accession #: Q13421 ELISA Kit: RayBio Human Mesothelin ELISA	MFRP Gene Symbol: MFRP Gene ID: 83552 Accession #: Q9BY79 ELISA Kit: RayBio Human MFRP ELISA	Neuropilin-2 Gene Symbol: NRP2 VEGF165R2 Gene ID: 8828 Accession #: O60462 ELISA Kit: RayBio Human Neuropilin-2 ELISA	Neurturin Gene Symbol: NRTN Gene ID: 4902 Accession #: Q99748 ELISA Kit: RayBio Human Neurturin ELISA
Nidogen-2 Gene Symbol: NID2 Gene ID: 22795 Accession #: Q14112 ELISA Kit: RayBio Human Nidogen-2 ELISA	Olfactomedin-2 (Noelin-2) Gene Symbol: OLFM2 NOE2 Gene ID: 93145 Accession #: O95897 ELISA Kit: RayBio Human Olfactomedin-2 ELISA	p53 Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	PD-ECGF (Thymidine Phosphorylase) Gene Symbol: TYMP ECGF1 Gene ID: 1890 Accession #: P19971 ELISA Kit: RayBio Human PD-ECGF ELISA	PDGF-C Gene Symbol: PDGFC SCDGF UNQ174/PRO200 Gene ID: 56034 Accession #: Q9NRA1 ELISA Kit: RayBio Human PDGF-C ELISA
Progranulin Gene Symbol: GRN Gene ID: 2896 Accession #: P28799 ELISA Kit: RayBio Human PGRN ELISA	Ret Gene Symbol: RET CDHF12 CDHR16 PTC RET51 Gene ID: 5979 Accession #: P07949 ELISA Kit: RayBio Human Ret ELISA	ROBO4 Gene Symbol: ROBO4 UNQ421/PRO3674 Gene ID: 54538 Accession #: Q8WZ75 ELISA Kit: RayBio Human ROBO4 ELISA	Semaphorin 6B Gene Symbol: SEMA6B SEMAN SEMAZ UNQ1907/PRO4353 Gene ID: 10501 Accession #: Q9H3T3 ELISA Kit: RayBio Human Semaphorin 6B ELISA	SERPINF1 (PEDF) Gene Symbol: SERPINF1 PEDF PIG35 Gene ID: 5176 Accession #: P36955 ELISA Kit: RayBio Human Serpin F1 ELISA
SREC-I (SCARF1) Gene Symbol: SCARF1 KIAA0149 SREC Gene ID: 8578 Accession #: Q14162 ELISA Kit: RayBio Human SREC-I ELISA	SREC-II (SCARF2) Gene Symbol: SCARF2 SREC2 SREPCR Gene ID: 91179 Accession #: Q96GP6 ELISA Kit: RayBio Human SREC-II ELISA	TLR1 Gene Symbol: TLR1 KIAA0012 Gene ID: 7096 Accession #: Q15399 ELISA Kit: RayBio Human TLR1 ELISA	TLR3 Gene Symbol: TLR3 Gene ID: 7098 Accession #: O15455 ELISA Kit: RayBio Human TLR3 ELISA	TREM-2 Gene Symbol: TREM2 Gene ID: 54209 Accession #: Q9NZC2 ELISA Kit: RayBio Human TREM-2 ELISA
Tripeptidyl-peptidase I (TPP1) Gene Symbol: TPP1 CLN2 GIG1 UNQ267/PRO304 Gene ID: 1200 Accession #: O14773 ELISA Kit: RayBio Human Tripeptidyl-peptidase I ELISA	TRKC Gene Symbol: NTRK3 TRKC Gene ID: 4916 Accession #: Q16288 ELISA Kit: RayBio Human TRKC ELISA	TROY (TNFRSF19) Gene Symbol: TNFRSF19 TAJ TROY UNQ1888/PRO4333 Gene ID: 55504 Accession #: Q9NS68 ELISA Kit: RayBio Human TROY ELISA	Uromodulin Gene Symbol: UMOD Gene ID: 7369 Accession #: P07911 ELISA Kit: RayBio Human Uromodulin ELISA	XIAP Gene Symbol: XIAP API3 BIRC4 IAP3 Gene ID: 331 Accession #: P98170 ELISA Kit: RayBio Human XIAP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q13 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

QAH-CYT-13.pdf

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