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規格

規格	22樣本、50樣本(產品加服務)、8樣本、22樣本(產品加服務)、8樣本(產品加服務)、50樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	PDGF-BB, PAI-1, TIMP-1, MMP-9, CHI3L1, E-Selectin, Ferritin, Pepsinogen 1, Pepsinogen 2, PSA-free, PSA-total, Cathepsin B / Pro-Cathepsin B, RANTES / CCL5, IL-6R, HE4 / WFDC2, ICAM-1 / CD54, Lipocalin-2 / NGAL, MIP-1 delta / CCL15, PARC / CCL18, VCAM-1 / CD106
靶標數	20

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Cathepsin B (Pro-Cathepsin B) Gene Symbol: CTSB CPSB Gene ID: 1508 Accession #: P07858 ELISA Kit: RayBio	CHI3L1 Gene Symbol: CTSB CPSB Gene ID: 1508 Accession #: P07858 ELISA Kit: RayBio	E-Selectin Gene Symbol: CHI3L1 Gene ID: 1116 Accession #: P36222 ELISA Kit: RayBio Human CHI3L1 ELISA	Ferritin Gene Symbol: SELE ELAM1 Gene ID: 6401 Accession #: P16581 ELISA Kit: RayBio Human E-Selectin ELISA	HE4 (WFDC2) Gene Symbol: FTH1 FTH FTHL6 OK/SW-cl.84 PIG15 Gene ID: 2495 Accession #: P02794 ELISA Kit: RayBio Human Ferritin ELISA
ICAM-1 (CD54) Gene Symbol: WFDC2 HE4 WAP5 Gene ID: 10406 Accession #: Q14508 ELISA Kit: RayBio Human HE4/WFDC2 ELISA	IL-6 R Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	Lipocalin-2 (NGAL) Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	MIP-1 delta (CCL15) Gene Symbol: LCN2 HNL NGAL Gene ID: 3934 Accession #: P80188 ELISA Kit: RayBio Human Lipocalin-2 ELISA	MMP-9 Gene Symbol: CCL15 MIP5 NCC3 SCYA15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA
PAI-1 Gene Symbol: MMP9 CLG4B Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA	PARC (CCL18) Gene Symbol: SERPINE1 PAI1 PLANH1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA	PDGF-BB Gene Symbol: CCL18 AMAC1 DCCK1 MIP4 PARC SCYA18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA	Pepsinogen 1 Gene Symbol: PDGFB PDGF2 SIS Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	Pepsinogen 2 Accession #: ELISA Kit: RayBio Human Pepsinogen I ELISA
PSA-free Gene Symbol: PGC Gene ID: 5225 Accession #: P20142 ELISA Kit: RayBio Human Pepsinogen II ELISA	PSA-total Gene Symbol: KLK3 APS Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA free ELISA	RANTES (CCL5) Gene Symbol: KLK3 APS Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA-total ELISA	TIMP-1 Gene Symbol: TIMP1 CLGI TIMP Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 L1CAM Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cancer Discovery Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相關產品

Anti-Gus

Anti-Xa21

Anti-HPT

Anti-CP4-EPSPS

文件

QAH-CDA-1.pdf

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