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規格

規格	22樣本(產品加服務)、8樣本、22樣本、50樣本(產品加服務)、8樣本(產品加服務)、50樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	Angiopoietin-1, IL-10, IL-4, VEGF-A, GM-CSF, IL-12 p40, TGF alpha, IL-12 p70, CXCL16, Angiostatin, EGF, GCSF, MMP-1, MMP-9, uPAR, VEGF-D, VEGFR2, VEGFR3, TGF beta 3, FGF-4, Follistatin, Tie-1, IL-1 beta / IL-1F2, Tie-2 / TEK, PECAM-1 / CD31, MCP-2 / CCL8, MCP-3 / MARC / CCL7, I-309 / TCA-3 / CCL1, I-TAC / CXCL11, MCP-4 / CCL13
靶標數	30

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiostatin Gene Symbol: PLG Gene ID: 5340 Accession #: P00747 ELISA Kit: RayBio Human Angiostatin ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA
Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA
IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA
MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956 ELISA Kit: RayBio Human MMP-1 ELISA	MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA
PECAM-1 (CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284 ELISA Kit: RayBio Human PECAM-1 ELISA	TGF alpha Gene Symbol: TGFA Gene ID: 7039 Accession #: P01135 ELISA Kit: RayBio Human TGF-alpha ELISA	TGF beta 3 ?	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590 ELISA Kit: RayBio Human Tie-1 ELISA	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Tie-2 ELISA
uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human VEGF R3 ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Angiogenesis Array Q3 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相關產品

Anti-Gus

Anti-Xa21

Anti-HPT

Anti-CP4-EPSPS

文件

QAH-ANG-3.pdf

文獻(1)

Serum differentially expressed angiogenic cytokines in head and neck vascular malformations,Yuichiro Yamamoto, Tetsuya Inoue, Miyu Inoue, Mana Murae, Masayoshi Fukasawa, Mika K. Kaneko, Yukinari Kato, Kohji Noguchi,INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ,(IF:6.208)
Species：	Human
Sample Type：	serum

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Product Features

Target Names

Application Notes

Storage/Stability