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規格

規格	50樣本(產品加服務)、22樣本(產品加服務)、8樣本(產品加服務)、8樣本、22樣本、50樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	IFN-gamma, TNF alpha, IGF-1, IL-10, VEGF-A, IGFBP-2, IL-12 p40, IL-6, PDGF-BB, Resistin, AgRP, Growth Hormone, IGFBP-1, IL-12 p70, Insulin, Leptin, MSP alpha/beta, PAI-1, Somatotropin, TGF beta 1, BDNF, Chemerin, Pepsinogen 1, Pepsinogen 2, Procalcitonin, Prolactin, RBP4, Thrombospondin-1, IL-8 / CXCL8, RANTES / CCL5, TNF R1 / TNFRSF1A, IL-1 beta / IL-1F2, Angiopoietin-like protein 4, Adipsin / Complement Factor D, SAA / Serum Amyloid A, TNF R2 / TNFRSF1B, IL-1ra / IL-1RN / IL-1 F3 / IRAP, Adiponectin / ACRP30, CRP / C-Reactive Protein, Lipocalin-2 / NGAL, Osteoprotegerin / TNFRSF11B
靶標數	40

詳細描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 μL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA
Chemerin Gene Symbol: RARRES2 Gene ID: 5919 Accession #: Q99969 ELISA Kit: RayBio Human Chemerin ELISA	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA
IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	Insulin Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human Insulin ELISA
Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	Lipocalin-2 (NGAL) Gene Symbol: LCN2 Gene ID: 3934 Accession #: P80188 ELISA Kit: RayBio Human Lipocalin-2?ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PAI-1 Gene Symbol: SERPINE1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA
PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	Pepsinogen 1 Gene Symbol: PGA3/PGA4/PGA5 ELISA Kit: RayBio Human Pepsinogen I ELISA	Pepsinogen 2 Gene Symbol: PGC Gene ID: 5225 Accession #: P20142 ELISA Kit: RayBio Human Pepsinogen II ELISA	Procalcitonin Gene Symbol: CALCA Gene ID: 796 Accession #: P01258 ELISA Kit: RayBio Human Procalcitonin ELISA	Prolactin Gene Symbol: PRL Gene ID: 5617 Accession #: P01236
RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	RBP4 Gene Symbol: RBP4 Gene ID: 5950 Accession #: P02753 ELISA Kit: RayBio Human RBP-4 ELISA	Resistin Gene Symbol: RETN Gene ID: 56729 Accession #: Q9HD89 ELISA Kit: RayBio Human Resistin ELISA	SAA (Serum Amyloid A) Gene Symbol: SAA1 Gene ID: 6288 Accession #: P0DJI8 ELISA Kit: RayBio Human SAA ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA
Thrombospondin 1 Gene Symbol: THBS1 Gene ID: 7057 Accession #: P07996 ELISA Kit: RayBio Human Thrombospondin 1 ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Obesity Array Q3 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相關產品

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文件

QAH-ADI-3.pdf

文獻(3)

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies,Yadav Tanuja T., Patil Piyush D., Shaikh Gulam Moin, Kumar Maushmi S., Chintamaneni Meena, YC Mayur,3 Biotech ,(IF:2.8)
Species：	Human
Sample Type：	plasma

View publications

Lipoaspirate fluid derived factors and extracellular vesicles accelerate wound healing in a rat burn model,Jiaxu Zhou, Yilan Song, Xingmei Wang, Xinrui Li, Chang Liu, Chenchen Tian, Chongyang Wang, Liangchang Li, Guanghai Yan, Hong Cui,Pharmaceuticals ,(IF:4.6) 作者單位：延邊大學
Species：	Human
Sample Type：	unknown

View publications

Reversion of breast epithelial polarity alterations caused by obesity,Ravel Ray, Stuti Goel, Hind Al Khashali, Ban Darweesh, Ben Haddad, Caroline Wozniak, Robert Ranzenberger, Jeneen Khalil, Jeffrey Guthrie, Deborah Heyl, Hedeel Guy Evans,Biomedicines ,(IF:4.7)
Species：	Human
Sample Type：	serum

View publications

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Product Features

Target Names

Application Notes

Storage/Stability