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規(guī)格

規(guī)格	2樣本(產(chǎn)品加服務(wù))、8樣本(產(chǎn)品加服務(wù))、4樣本、8樣本、4樣本(產(chǎn)品加服務(wù))、2樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Mouse
檢測靶標(biāo)	bFGF, IGF-1, IL-2, VEGF-A, GM-CSF, HGF, IGFBP-2, IGFBP-3, M-CSF, EGF, GCSF, VEGF-D, VEGFR3, IGF-2, IGFBP-5, IGFBP-6, Amphiregulin, IL-7, VEGFR1, SCF / KITLG
靶標(biāo)數(shù)	20

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Amphiregulin Gene Symbol: AREG SDGF; AREGB Gene ID: 11839 Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA	bFGF Gene Symbol: FGF2 Gene ID: 14173 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	EGF Gene Symbol: EGF Gene ID: 13645 Accession #: P01132 ELISA Kit: RayBio Mouse EGF ELISA	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 12985 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 12983 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA
HGF Gene Symbol: MET Gene ID: 15234 Accession #: Q08048 ELISA Kit: RayBio Mouse HGF ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 16000 Accession #: P05017 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: IGF2 PP1446 Gene ID: 16002 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IGFBP-2 Gene ID: 16008 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 IBP3 Gene ID: 16009 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA
IGFBP-5 Gene Symbol: IGFBP5 IBP5 Gene ID: 16011 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 IBP6 Gene ID: 16012 Accession #: P47880 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IL-2 Gene Symbol: IL2 IL-2 Gene ID: 16183 Accession #: P04351 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 16196 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 12977 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA
SCF Gene Symbol: KITLG SCF Gene ID: 17311 Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 22339 Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF ELISA	VEGF-D Gene Symbol: FIGF VEGFD Gene ID: 14205 Accession #: P97946	VEGFR1 Gene Symbol: FLT1 FLT FRT VEGFR1 Gene ID: 14254 Accession #: P35969 ELISA Kit: RayBio Mouse VEGF R1 ELISA	VEGFR3 Gene Symbol: FLT4 VEGFR3 Gene ID: 14257 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Growth Factor Antibody Array C2 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAM-GF-2.pdf

文獻(1)

A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes,Saad Entsar A., Zahran Faten, El-Ablack Fawzia Z., Eleneen Ahmed M. Abo,APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ,(IF:3.094)
Species：	Mouse
Sample Type：	liver tissue

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Product Features

Target Names

Application Notes

Storage/Stability