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規格

規格	2樣本、8樣本、4樣本(產品加服務)、8樣本(產品加服務)、2樣本(產品加服務)、4樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Mouse
檢測靶標	bFGF, IGF-1, VEGF-A, GM-CSF, HGF, M-CSF, EGF, GCSF, IGF-2, SCF / KITLG
靶標數	10

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bFGF Gene Symbol: FGF2 Gene ID: 14173 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	EGF Gene Symbol: EGF Gene ID: 13645 Accession #: P01132 ELISA Kit: RayBio Mouse EGF ELISA	GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 12985 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 12983 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	HGF Gene Symbol: MET Gene ID: 15234 Accession #: Q08048 ELISA Kit: RayBio Mouse HGF ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 16000 Accession #: P05017 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGF-2 Gene Symbol: IGF2 PP1446 Gene ID: 16002 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 12977 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	SCF Gene Symbol: KITLG SCF Gene ID: 17311 Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 22339 Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Growth Factor Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAM-GF-1.pdf

文獻(2)

Implication of the Autophagy-Related Protein Beclin1 in the Regulation of EcoHIV Replication and Inflammatory Responses,Yi Han, Yan-Yu Zhang, Yi-Qian Pan, Xiao-Jun Zheng, Kun Liao, Hai-Yu Mo, Hui Sheng, Qi-Nian Wu, Ze-Xian Liu, Zhao-Lei Zeng, Wei Yang, Shu-Qiang Yuan, Peng Huang, Huai-Qiang Ju, Rui-Hua Xu,MOLECULAR CELL ,(IF:16) 作者單位：中山大學附屬腫瘤醫院(中山大學腫瘤防治中心)
Species：	Mouse
Sample Type：	brain

View publications

Nematode-Induced Growth Factors Related to Angiogenesis in Autoimmune Disease Attenuation,Wenhao Yang, Lina Chen, Juncen Guo, Fang Shi, Qingxin Yang, Liang Xie, Danli Lu, Yingna Li, Jiaxin Luo, Li Wang, Li Qiu, Ting Chen, Yan Li, Rui Zhang, Lu Chen, Wenming Xu, Hanmin Liu,Cells ,(IF:7.666) 作者單位：四川大學
Species：	Mouse
Sample Type：	mucosa

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Product Features

Target Names

Application Notes

Storage/Stability