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規格

規格	4樣本、4樣本(產品加服務)、8樣本(產品加服務)、8樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Mouse
檢測靶標	IFN-gamma, TNF alpha, bFGF, IL-13, VEGF-A, GM-CSF, IL-6, IL-12 p70, Leptin, M-CSF, TIMP-1, TIMP-2, GCSF, IGF-2, IL-9, IL-12 p40/p70, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, IL-1 beta / IL-1F2, MIG / CXCL9, IL-1 alpha / IL-1F1, MCP-1 / CCL2, Platelet Factor 4 / CXCL4, Thrombopoietin / TPO
靶標數	24

詳細描述

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA
IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGF-2 Gene Symbol: Igf2 Accession #: P09535 ELISA Kit: RayBio Mouse IGF-2 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P43432 ELISA Kit: RayBio Mouse IL-12 p40/70 ELISA
IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA	TIMP-1 Gene Symbol: TIMP1 Accession #: P12032 ELISA Kit: RayBio Mouse TIMP-1 ELISA
TIMP-2 Gene Symbol: TIMP2 Accession #: Q6GXA8 ELISA Kit: RayBio Mouse TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Mouse Angiogenesis Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAM-ANG-G1.pdf

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