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規格
| 規格 | 4 樣本、8 樣本 |
| 樣本類型 | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| 原理方法 | Sandwich-based |
| 檢測方法 | Fluorescence Laser Scanner |
| 定量與否 | Semi-Quantitative |
| 載體 | Glass Slide |
| 適用種屬 | Human |
| 檢測靶標 | Cystatin C, Resistin, Trappin-2, 4-1BB, ACE-2, Adipsin, ANGPTL4, CRP, PAI-1, Albumin, Clusterin, FABP1, Osteopontin, TFF3, TIM-1, AMPKa1, p53, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IGFBP-5, Livin, p21, SMAC, Survivin, XIAP, p27, 2B4, A1BG, A2M, ABL1, ACE, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, Afamin, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha Lactalbumin, ALPP, AMICA, Amylin, ANGPTL3, Annexin A7, APC, APCS, Apelin, APEX1, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, ASPH, Attractin, B3GNT1, BAF57, BAFF, BCAM, Beta Defensin 4, beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, BTK, C-Peptide, C3a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chymase, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC3B, CNDP1, COCO, Complement Factor H, Contactin-1, Contactin-2, COX-2, CPN2, Creatinine, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, Cytokeratin 8, D-Dimer, DBI, DCBLD2, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endothelin Receptor A, Enolase 2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP2, FABP3, FABP4, FAK, FAP, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FOXO1, FoxP3, FRK, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP32, HSP40, HSP90, HSPA8, IBSP, IGF2BP1, IL-33, IL-34, INSL3, INSRR, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, LOX-1, LPS, LRG1, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Omentin, Osteocalcin, OX40, P-Cadherin, PAK7, Pancreastatin, Pancreatic Polypeptide, Park7, PCAF, PD-1, PDX-1, PEDF, PGRP-S, PI 16, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Pro-Cathepsin B, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, Procalcitonin, Progesterone, Prohibitin, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Ret, RIP1, ROCK1, ROCK2, ROR1, ROR2, Ryk, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SNCG, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, TEC, TFF1, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, TYK2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, ZAG, ZAP70, 11b-HSD1, ADAMTS-L2, AFP, Alpha 1 AG, Alpha 1 Microglobulin, APN, APP, ARB1, BAI-1, Beta 2M, C1qTNF1, C2, C5/C5a, C7, C8b, C9, CBG, Chromogranin A, CLEC14A, CPA1, Cytokeratin18, Cytokeratin19, DEFA1/3, Endorphin Beta, ENPP2, Factor XIII B, Fc gamma RIIIB, Fcg RIIB/C, Gas1, GRP75, hCGb, IDUA, IL-28B, Integrin alpha V, LTF, Nesfatin, Ntn1, OCT3/4, Pappalysin-1, PEPSINOGEN I, PEPSINOGEN II, PI 3Kinase p85 alpha, pro-Glucagon, ROS, S-100b, S100A4, S100A6, S100A8, SCGF, VEGF R1, Vitamin D-BP |
| 靶標數 | 493 |
詳細描述
Protein S-Nitrosylation plays an unusually prominent role in cell signaling, development and growth. The RayBio? G-Series Human Protein S-Nitrosylation Antibody Array 2 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein S-Nitrosylation of cysteine and be used to monitor the activation or function of important biological pathways.
RayBiotech is committed to develop a series of S-Nitrosylation antibody arrays. This multiplex glass slide array is specifically designed for simultaneous identification of the relative levels of S-Nitrosylation of cysteine of 493 different human proteins in cell lysate, culture supernatant, serum, plasma and other biological samples. By monitoring the changes in protein S-Nitrosylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
RayBio? S-Nitrosylation Detection Kit uses a modified 'biotin-switch' method (Jaffrey et al.) to allow for the direct visualization of S-nitrosylated proteins by western blot analysis as well as by antibody array. In this S-nitrosylation biotin switch assay, unmodified free cysteines are first blocked. S-nitrosylated cysteines are then selectively reduced for specific labeling with biotin-maleimide reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Biotinylation of the newly formed thiol groups can then be detected by western blot or antibody array. In addition, avidin resin can be used to selectively enrich S-nitrosylated proteins/peptides labeled with biotin. The biotin labeled sample is added into antibody array glass slide wells. The antibody array slide wells are washed. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
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