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規(guī)格

規(guī)格	4樣本(產(chǎn)品加服務)、4樣本、8樣本、8樣本(產(chǎn)品加服務)
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	VEGFR2, Alpha-fetoprotein, CD38, E-Cadherin, GATA-4, hCG beta, Nanog, Nestin, Oct-4, SOX17, SOX2, Neprilysin-2 / MMEL1, PDX-1 / IPF1, BMPR-IA / ALK-3, ALK-6 / BMPR-IB, Brachyury / Protein T
靶標數(shù)	15

詳細描述

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Alpha-fetoprotein Gene Symbol: AFP HPAFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	OCT-4 Gene Symbol: POU5F1 OCT3 OCT4 OTF3 Gene ID: 5460 Accession #: Q01860 ELISA Kit: RayBio Human OCT-4 ELISA	BMPR-IA (ALK-3) Gene Symbol: BMPR1A ACVRLK3 ALK3 Gene ID: 657 Accession #: P36894 ELISA Kit: RayBio Human BMPR-IA ELISA	ALK-6 (BMPR-IB) Gene Symbol: BMPR1B Gene ID: 658 Accession #: O00238 ELISA Kit: RayBio Human ALK-6 ELISA	Brachyury (Protein T) Gene Symbol: T Gene ID: 6862 Accession #: O15178
CD38 Gene Symbol: CD38 Gene ID: 952 Accession #: P28907 ELISA Kit: RayBio Human CD38 ELISA	E-Cadherin Gene Symbol: CDH1 CDHE UVO Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	GATA4 Gene Symbol: GATA4 Gene ID: 2626 Accession #: P43694 ELISA Kit: RayBio Human GATA4 ELISA	hCG beta Gene Symbol: CGB CGB3; CGB5; CGB7; CGB8 Gene ID: 1082; 93659; 94027; 94115 Accession #: P01233 ELISA Kit: RayBio Human hCG-beta ELISA	Nanog Gene Symbol: NANOG Gene ID: 79923 Accession #: Q9H9S0 ELISA Kit: RayBio Human Nanog ELISA
Nestin Gene Symbol: NES Nbla00170 Gene ID: 10763 Accession #: P48681 ELISA Kit: RayBio Human Nestin ELISA	PDX-1 (IPF1) Gene Symbol: PDX1 IPF1 STF1 Gene ID: 3651 Accession #: P52945 ELISA Kit: RayBio Human PDX-1 ELISA	SOX2 Gene Symbol: SOX2 Gene ID: 6657 Accession #: P48431 ELISA Kit: RayBio Human SOX2 ELISA	SOX17 Gene Symbol: SOX17 Gene ID: 64321 Accession #: Q9H6I2 ELISA Kit: RayBio Human SOX17 ELISA	VEGFR2 Gene Symbol: KDR FLK1 VEGFR2 Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Stem Cell Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相關產(chǎn)品

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文件

AAH-SC-G1.pdf

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