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規格

規格	4樣本、8樣本(產品加服務)、8樣本、4樣本(產品加服務)、2樣本(產品加服務)、2樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標	VEGFR2, Alpha-fetoprotein, CD38, E-Cadherin, GATA-4, hCG beta, Nanog, Nestin, Oct-4, SOX17, SOX2, Neprilysin-2 / MMEL1, PDX-1 / IPF1, BMPR-IA / ALK-3, ALK-6 / BMPR-IB, Brachyury / Protein T
靶標數	15

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Alpha-fetoprotein Gene Symbol: AFP HPAFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	BMPR-IA (ALK-3) Gene Symbol: BMPR1A ACVRLK3 ALK3 Gene ID: 657 Accession #: P36894 ELISA Kit: RayBio Human BMPR-IA ELISA	ALK-6 (BMPR-IB) Gene Symbol: BMPR1B Gene ID: 658 Accession #: O00238 ELISA Kit: RayBio Human ALK-6 ELISA	Brachyury (Protein T) Gene Symbol: T Gene ID: 6862 Accession #: O15178	CD38 Gene Symbol: CD38 Gene ID: 952 Accession #: P28907 ELISA Kit: RayBio Human CD38 ELISA
E-Cadherin Gene Symbol: CDH1 CDHE UVO Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA	GATA4 Gene Symbol: GATA4 Gene ID: 2626 Accession #: P43694 ELISA Kit: RayBio Human GATA4 ELISA	hCG beta Gene Symbol: CGB CGB3; CGB5; CGB7; CGB8 Gene ID: 1082; 93659; 94027; 94115 Accession #: P01233 ELISA Kit: RayBio Human hCG-beta ELISA	Nanog Gene Symbol: NANOG Gene ID: 79923 Accession #: Q9H9S0 ELISA Kit: RayBio Human Nanog ELISA	Nestin Gene Symbol: NES Nbla00170 Gene ID: 10763 Accession #: P48681 ELISA Kit: RayBio Human Nestin ELISA
OCT-4 Gene Symbol: POU5F1 OCT3 OCT4 OTF3 Gene ID: 5460 Accession #: Q01860 ELISA Kit: RayBio Human OCT-4 ELISA	PDX-1 (IPF1) Gene Symbol: PDX1 IPF1 STF1 Gene ID: 3651 Accession #: P52945 ELISA Kit: RayBio Human PDX-1 ELISA	SOX17 Gene Symbol: SOX17 Gene ID: 64321 Accession #: Q9H6I2 ELISA Kit: RayBio Human SOX17 ELISA	SOX2 Gene Symbol: SOX2 Gene ID: 6657 Accession #: P48431 ELISA Kit: RayBio Human SOX2 ELISA	VEGFR2 Gene Symbol: KDR FLK1 VEGFR2 Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process

Kit Components

Human Stem Cell Array C1 Membranes(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

相關產品

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Anti-CP4-EPSPS

文件

AAH-SC-1.pdf

文獻(1)

SPC25 promotes proliferation and stemness of hepatocellular carcinoma cells via the DNA-PK/AKT/Notch1 signaling pathway,Sara Verdura, Jose Antonio Encinar, Eduard Teixidor, Antonio Segura-Carretero, Vicente Micol, Elisabet Cuyàs, Joaquim Bosch-Barrera, Javier A. Menendez,Cancers ,(IF:6.575)
Species：	Human
Sample Type：	unknown

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Product Features

Target Names

Application Notes

Storage/Stability