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規格

規格	4 樣本、8 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	Growth Hormone, bad, Bcl-w, BID, Cytochrome C, alpha-Synuclein, DAN, FSH, GRP, 14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, ABAT, ABCF1, ABI3BP, ACAA, ACAA1, ACAA2, ACACA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK, Ahsp, AIF, AK2, AKAP9, AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1, ALDH1A1, ALDH9A1, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha Tubulin, AlphaA Crystallin, ALS, ALS+B62:B1212CR1, Als2, Androgen Receptor, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, ANP, APA, APM2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, ARX, ASH2L, Asialoglycoprotein Receptor 2, ASK1, Aspartate Aminotransferase, Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, Band 3, BASP1, Bassoon, BAZ2B, BCHE, BCOR, beta 1 Spectrin, beta B1 Crystallin, BIN2, BIRC6, BLMH, BLVRB, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C3orf75, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150, CA2, CA3, CACNB4, CAD, Cadherin-22, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1, Calpastatin, Calretinin, Calumenin, CAP1, CapG, CAPZA1, CARHSP1, Caspase-14, Catalase, Catenin gamma, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD16, CD32, CD35, CD41, CD5L, CD9, CD98, CDA, CDC5L, CDK2, CEACAM-8, CECR1, CENPF, CEP57, CETP, Cezanne, CFHR 1, CFHR4, CFI, CFL1, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, CLPS, CLTA, CNN2, CNOT1, CO4A2, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, COG4, COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Complement Factor B, COPS8, Corneodesmosin, Coronin 3, Cortactin, COTL1, CPEB3, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin B, Cystatin D, Cystatin E/M, Cystatin SN, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin, DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, ECHS1, ECM-1, EEF1G, EEF2, EFEMP2, EFTUD2, EHD1, EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1, EMILIN1, EMSY, EN2, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, F-box protein 38, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, FBPase 1, FBPase 2, FCGBP, FDPS, FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP, G6PD, GALNT2, GAPHD, GARNL1, GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC, GCSH, GDA, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLPH4, GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GSTM1, GSTO1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, hHR23b, HIBADH, HINT1, HIP1R, Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha, hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G, hnRNP L, hnRNP M1-M4, hnRNP U, Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRG, HPRT, HRSP12, HSC70, HSP47, LAMR1, Tubulin beta-1 chain, Tubulin beta-3 chain, Ubiquitin-activating enzyme E1, Cyclophilin A, Cystatin S, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Aminopeptidase??PILS / ARTS1 / ERAP1, Cathepsin Z / Cathepsin X / Cathepsin P, Coagulation Factor VII / F7, Coagulation Factor XI / F11 / PTA, Carboxypeptidase M / CPM, DEP-1 / CD148, Human Amyloid-like protein 1 / APLP-1, Apolipoprotein(a), B7-H3 / CD276, Cadherin-6 / KCAD, Collectin-12 / CL-P1, Contactin-3 / BIG-1, FABP5 / E-FABP, CD155 / PVR, Aminoacylase-1 / ACY1, Alkaline Phosphatase / ALPL, Annexin A5 / Annexin V, Aminopeptidase LRAP / ERAP2, Antithrombin 3 / Serpin C1, B7-H2 / ICOS Ligand, C1S / Complement??Component??C1s, C4.4A / LYPD3, Carboxypeptidase E / CPE, Carboxypeptidase B2 / CPB2, CD21 / CR2 / EBV receptor, CD39L4 / ENTPD5, CD42b / GPIb-alpha, CD48 / SLAMF2, Carboxylesterase 1 / CES1, Complement Factor H-related 5 / CFHR5, Cytokeratin 20 / KRT20, Endorepellin / Perlecan, GOLM1 / GP73, GPV / CD42d, Thrombospondin 5 / COMP, ADAM DEC1, Angiopoietin-like protein 6, Angiopoietin-like protein 8, BMP-1 / PCP, GDF-7 / BMP-12, Apolipoprotein A-I-binding protein / APOA1BP, Apolipoprotein F / ApoF, Apolipoprotein L1 / ApoL1, Apolipoprotein L2 / ApoL2
靶標數	507

詳細描述

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, LAMR1, ABAT, ABCF1, ABI3BP, ACAA1, ACAA2, ACACA, ACAA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAMDEC1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK , Ahsp, AIF, AK2 , AKAP9 , AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1 , ALDH1A1, ALDH9A1, Alkaline Phosphatase, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha-synuclein, alpha Tubulin, AlphaA Crystallin, ALS, Als2 , ALS+B62:B1212CR1 , Aminoacylase-1, Androgen Receptor, ANGPTL6, ANGPTL8, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, Annexin V, ANP, Antithrombin 3, APA, APLP-1, APM2, Apo (a), APOA1BP, ApoF, ApoL1, ApoL2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, Aminopeptidase PILS, ARX, ASH2L , Asialoglycoprotein Receptor 2, ASK1 , Aspartate Aminotransferase , Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, B7-H2, B7-H3, bad, Band 3, BASP1, Bassoon, BAZ2B, BCHE, bcl-w, BCOR, beta 1 Spectrin , beta B1 Crystallin, Tubulin beta-1 chain, Tubulin beta-3 chain, BID, BIN2, BIRC6, BLMH, BLVRB, BMP-1, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C1S, C3orf75, C4.4A, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150 , CA2, CA3, CACNB4, CAD , Cadherin-22, Cadherin-6, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1 , Calpastatin, Calretinin, Calumenin , CAP1, CapG, CAPZA1, Carboxypeptidase B2, CARHSP1, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, Cathepsin Z, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD155, ADP-Ribosyl Cyclase 2, CD16, CD21, CD32, CD35, CD39L4, CD41, CD42b, CD48, CD5L, CD9 , CD98, CDA, CDC5L, CDK2 , CEACAM-8, CECR1, CENPF, CEP57, Carboxylesterase 1, CETP, Cezanne, CFHR 1, CFHR4, Complement Factor H-related 5, CFI, CFL1, Coagulation Factor VII, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, Collectin-12, CLPS, CLTA, CNN2, CNOT1, CO4A2, COG4 , COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Thrombospondin 5, Complement Factor B, Contactin-3, COPS8 , Corneodesmosin, Coronin 3, Cortactin, COTL1, Carboxypeptidase E, CPEB3, Carboxypeptidase M, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin A, Cyclophilin B, Cystatin D, Cystatin E/M, Cystatin S, Cystatin SN, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 20, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DAN, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, DEP-1, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin , DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, Ubiquitin-activating enzyme E1, ECHS1 , ECM-1, EEF1G , EEF2, EFEMP2 , EFTUD2, EHD1 , EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1 , EMILIN1 , EMSY, EN2, Endorepellin, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, Aminopeptidase LRAP, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, Coagulation Factor XI, FABP5, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, F-box protein 38, FBPase 2, FBPase 1, FCGBP, FDPS , FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FSH, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP , GALNT2, Catenin gamma, GAPHD, GARNL1 , GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC , GCSH , GDA, GDF7, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, G6PD, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma , GSTO1, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, GPV, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLM1, GOLPH4 , GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GRP, GSTM1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, Growth Hormone, hHR23b, HIBADH , HINT1, HIP1R , Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha , hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G , hnRNP L, hnRNP M1-M4 , hnRNP U , Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRT, HPRG, HRSP12, HSC70, HSP47

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Protein Tyrosine Phosphorylation Antibody Array G3 Slide(s)
2X Cell Lysis Buffer
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail Set II
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
Biotin-Conjugated Anti-phosphotyrosine
Fluorescent dye-Conjugated Streptavidin
Wash Buffer III
Adhesive film

Other Materials Required

Shaker
Laser scanner for fluorescence detection
Aluminum foil
Distilled water
Plastic box
50 ml Centrifuge tube
Isopropanol (2-propanol)

Protocol Outline

Dry the Glass Slide
Blocking and Incubation
Fluorescence Detection

Storage/Stability

Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.

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文件

AAH-PTYR-G3.pdf

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