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規格

規格	4 樣本、8 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	Cystatin C, Resistin, Trappin-2, ACE-2, PAI-1, Albumin, Clusterin, FABP1, AMPKa1, p53, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IGFBP-5, Livin, p21, SMAC, XIAP, p27, A1BG, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, ADAMTSL-2, Afamin, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha Lactalbumin, Alpha-fetoprotein, ALPP, AMICA, Amylin, Annexin A7, APC, APCS, Apelin, ASPH, Attractin, B3GNT1, BAF57, BCAM, Beta Defensin 4, beta IG-H3, Biglycan, BMP-9, BMX, BNIP2, BNP, BTK, c-Abl, C-Peptide, C3a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, CART, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD38, CD45, CD46, CD47, CD59, CD61, CD71, CD74, CD79 alpha, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chymase, CK-MB, Claudin-3, Claudin-4, COCO, Complement Factor H, Contactin-1, CPN2, Creatinine, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, D-Dimer, DBI, Desmin, DLL1, DLL4, DMP-1, E-Cadherin, Endothelin Receptor A, Enolase 2, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP2, FABP3, FAK, FAP, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FOXO1, FoxP3, FRK, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas 1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, Hck, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP40, HSP90, HSPA8, IBSP, IL-34, IMP1, INSL3, INSRR, Integrin alpha-5, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, LOX-1, LPS, LRG1, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nestin, NET1, Netrin G2, Netrin-1, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Omentin, Osteocalcin, P-Cadherin, PAK7, Pancreastatin, PCAF, PD-1, PGRP-S, PI 16, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, Procalcitonin, Progesterone, Prohibitin, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, PSP, PTH, PTHLP, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Ret, RIP1, ROCK1, ROCK2, ROR1, ROR2, Ryk, S100 A6, S100 A8, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A12, Serpin A4, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SNCG, SOST, SOX17, SOX2, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, SYK, Syndecan-1, Syndecan-3, TAF4, TEC, TFF1, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, TSH, TSLP, TXK, TYK2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VGF, VIP Receptor 2, Vitamin D Receptor, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, ZAG, ZAP70, 11b-HSD1, Alpha 1 AG, Alpha 1 Microglobulin, APN, APP, ARB1, BAI-1, Beta 2M, C1qTNF1, C2, C5/C5a, C7, C8b, C9, CBG, Chromogranin A, CLEC14A, Cytokeratin18, Cytokeratin19, DEFA1/3, Endorphin Beta, ENPP2, Factor XIII B, Fc gamma RIIIB, Fcg RIIB/C, GRP75, hCGb, IDUA, IL-28B, LTF, Nesfatin, OCT3/4, Pappalysin-1, PEPSINOGEN I, PEPSINOGEN II, PI 3Kinase p85 alpha, pro-Glucagon, ROS, S-100b, S100A4, VEGF R1, Vitamin D-BP, SPARC-Like 1, Cathepsin B / Pro-Cathepsin B, IL-33 / IL-1F11, Angiopoietin-like protein 3, Angiopoietin-like protein 4, Apolipoprotein A-I / ApoA1, Apolipoprotein A-II / ApoA2, Apolipoprotein A-IV / ApoA4, Apolipoprotein B / ApoB, Apolipoprotein B-100 / Apo B-100, Apolipoprotein C-I / ApoC1, Apolipoprotein C-II / ApoC2, Apolipoprotein C-III / ApoC3, Apolipoprotein D / ApoD, Apolipoprotein E / ApoE, Apolipoprotein E-III / ApoE3, Apolipoprotein H / ApoH, BLAME / SLAMF8, CK beta 8-1 / CCL23, Coagulation Factor II / Thrombin / F2, HSP32 / HO-1 / HMOX1, PDX-1 / IPF1, CD90 / Thy1, Adipsin / Complement Factor D, Angiotensinogen / Serpin A8, CD44 / Heparan Sulfate Proteoglycan, CLEC3B / Tetranectin, Carnosine Dipeptidase-1 / CNDP1, Contactin-2 / TAG1, SCGF / CLEC11a, CD36 / SR-B3, TROP1 / EpCAM, Serpin A1 / Alpha-1-Antitrypsin, Serpin F1 / PEDF, 2B4 / CD244, ACE / CD143, APE1 / APEX1, Carboxypeptidase A1 / CPA1, CD26 / DPPIV, CD55 / DAF, Calsyntenin-1 / Alcadein-alpha, COX-2 / PTGS2, CRP / C-Reactive Protein, DCBLD2 / ESDN, FABP4 / A-FABP, HE4 / WFDC2, Cytokeratin 8 / KRT8, Osteopontin / SPP1, OX40 / TNFRSF4, Park7 / DJ-1, Pancreatic polypeptide / ProPPY / PP, Pleiotrophin / PTN, Serpin A3 / Alpha-1-Antichymotrypsin, Serpin A5 / Protein C Inhibitor, ADAM-17 / TACE, TFF3 / Trefoil Factor 3, TIM-1 / KIM-1, 4-1BB / TNFRSF9 / CD137, Trypsin 1 / PRSS1, Visfatin / PBEF1, ADAMTS-1 / METH1, Alpha-2 Microglobulin, BAFF / TNFSF13B, Survivin / BIRC5, Apolipoprotein M / ApoM
靶標數	493

詳細描述

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

11b-HSD1, 2B4, 4-1BB, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adipsin, Afamin, AFP, ALBUMIN, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, ARB1, ASPH, Attractin, B3GNT1, BAF57, BAFF, BAI-1, BCAM, Beta 2M, Beta Defensin 4, Beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, Btk, C1qTNF1, C2, C3a, C5/C5a, C7, C8b, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBG, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC14A, CLEC3B, Clusterin, CNDP1, COCO, Complement factor H, Contactin-1, Contactin-2, COX-2, CPA1, C-peptide, CPN2, Creatinine, CRP, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, DBI, DCBLD2, D-Dimer, DEFA1/3, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endorphin Beta, Endothelin Receptor A, Enolase 2, ENPP2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII B, FAK, FAP, Fc gamma RIIIB, Fcg RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FoxO1, FoxP3, FRK, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, hCGb, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, IBSP, IDUA, IGF2BP1, IGFBP-5, IL-28B, IL-33, IL-34, INSL3, INSRR, Integrin alpha V, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, Livin, LOX-1, LPS, LRG1, LTF, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Ntn1, OCT3/4, Omentin, Osteocalcin, Osteopontin, OX40, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARK7, P-Cadherin, PCAF, PD-1, PDX-1, PEDF, PEPSINOGEN I, PEPSINOGEN II, PGRP-S, PI 16, PI 3Kinase p85 alpha, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Prohibitin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Resistin, RET, RIP1, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100A4, S100A6, S100A8, S-100b, SART1, SART3, SCG3, SCGF, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SMAC, SNCG, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, Tec, TFF1, TFF3, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TIM-1, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, Trappin-2, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, Tyk2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VEGF R1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, XIAP, ZAG, ZAP70

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Tyrosine Phosphorylation Antibody Array G2 Slide(s)
2X Cell Lysis Buffer
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail Set II
Blocking Buffer
20X Wash Buffer I
20X Wash Buffer II
Biotin-Conjugated Anti-phosphotyrosine
Fluorescent dye-Conjugated Streptavidin
Wash Buffer III
Adhesive film

Other Materials Required

Shaker
Laser scanner for fluorescence detection
Aluminum foil
Distilled water
Plastic box
50 ml Centrifuge tube
Isopropanol (2-propanol)

Protocol Outline

Dry the Glass Slide
Blocking and Incubation
Fluorescence Detection

Storage/Stability

Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.

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文件

AAH-PTYR-G2.pdf

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