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規格

規格	2 樣本、4 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標	IFN-gamma, TNF alpha, Angiopoietin-1, bFGF, Decorin, IFN-beta, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-21, IL-4, LIF, VEGF-A, EGFR, ErbB3, Erythropoietin, Galectin-3, GM-CSF, HGF, IGFBP-2, IL-12 p40, IL-6, MMP-8, PDGF-BB, RAGE, TGF alpha, IL-5, IL-16, AgRP, Angiopoietin-2, FGF-6, Growth Hormone, IGF-1 R, IGFBP-1, IGFBP-3, IL-11, IL-12 p70, Insulin, Leptin, Leptin R, M-CSF, MIF, Oncostatin M, PDGF-AA, PDGF-AB, Somatotropin, TGF beta 1, TIMP-1, TIMP-2, TNFRSF27, XEDAR, CXCL16, Angiogenin, Angiostatin, EGF, Endostatin, GCSF, GRO alpha/beta/gamma, MMP-1, MMP-9, PLGF, uPAR, VEGF-D, VEGFR2, VEGFR3, bax, IGF-2, IGFBP-4, IGFBP-6, GDNF, TGF beta 2, TGF beta 3, Activin A, Activin B, Activin R2A, Activin R2A/B, ALK-1, Alpha Chain and Full Length, Amphiregulin, Angiopoietin-4, Apelin Receptor, APJ, APRIL, Artemin, AXL, BACE-1, BD-1, BDNF, beta-Catenin, beta-NGF, BIK, BLR-1, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPR-II, CCN2, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD163, CD91 Cluster II, Cerberus 1, CF10, Chem R23, CLC, CNTF, CNTF R alpha, CRAM-A/B, CRIM1, Cripto-1, CRTH-2, Cryptic, CSFR1, CSK, CTGF, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, D6, DAN, DANCE, DKK-1, Dkk-3, Dkk-4, Dtk, E-Selectin, EDA-A2, EDAR, EDG-1, EMAP-II, EML2, Endocan, Endothelin-1, Epiregulin, ErbB1, ErbB2, FACX, FADD, FAM3B, FGF R3, FGF R4, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-8, FGF-9, FGF-BP, FIL1 delta, FIL1 epsilon, FIL1 eta, FIL1 zeta, FLRG, Flt-3 Ligand, Follistatin, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Fusin, GDF-1, GDF-10, GDF-15, GDF-3, GDF-9, GFR alpha-2, GFR alpha-4, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF R alpha, Granzyme A, Grb2, Gremlin-1, Growth Hormone R, HB-EGF, HCR, ICAM-5, IDE, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-gamma R1, IGF-2 R, IL-17B, IL-17C, IL-17D, IL-17F, IL-19, IL-20, IL-22, IL-22BP, IL-23, IL-24, IL-27, IL-3, IL-31, IL-7, IL-9, Insulysin, ITGAL, Kremen-1, Kremen-2, LBP, LCK, LECT2, Lefty-A, LFA-1 alpha, LIF R alpha, Lipocalin-1, LRP-1 Cluster II, LRP-6, LTBP1, Lymphotoxin beta, M-CSF R, MADH9, MFG-E8, MFRP, MICA, MIP-2, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MSP alpha, MT3-MMP, MT5-MMP, MT6-MMP, MuSK, Neuritin, NeuroD1, Neuropilin-2, Neurturin, Nidogen-1, Nr-CAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin-A, Orexin-B, Osteocrin, P-Selectin, PDGF R alpha, PDGF R beta, PDGF-C, PDGF-D, Peroxiredoxin-6, Persephin, PLUNC, Prdx6, Progranulin, Prolactin, RELM beta, ROBO4, S100 A10, S100 A8/A9, sFRP-1, sFRP-3, sFRP-4, SIGIRR, SLPI, SMAD1, SMAD4, SMAD5, SMAD7, SMAD8, SMAD9, Spinesin, TCCR, TFPI, TGF beta R2, TGF beta R3, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin isform alpha, Thyroid Peroxidase, Tie-1, TIMP-3, TIMP-4, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF2, TNFRSF12A, TNFSF15, TNFSF3, TP, TPX, TRADD, TREM-1, TSG-6, TSLP R, TYRO3, Ubiquitin+1, uPA, Vasorin, VEGF-B, VEGF-C, WIF-1, WSX-1, IL-17RA, IL-17RB, IL-17RD, IL-23R, IL-4R, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, Activin R1A / ALK-2, CTLA-4 / CD152, BLC / CXCL13, NCAM-1 / CD56, TNF R1 / TNFRSF1A, IL-1 beta / IL-1F2, CD40 Ligand / TNFSF5, MIG / CXCL9, MIP-1 beta / CCL4, Angiopoietin-like protein 2, Angiopoietin-like protein 7, CD27 / TNFRSF7, VE-Cadherin / CDH5, Chordin-like 2 / CHRDL2, CXCL2 / CINC-3 / MIP2, Coagulation Factor X / F10, Follistatin-like 1 / FSTL1, GFR alpha-3 / GDNF R alpha-3, IL-10R alpha, IL-10R beta, IL-12R beta 1, IL-12R beta 2, IL-13R alpha 2, IL-15R alpha, IL-17RC, IL-18BPa, IL-38 / IL-1F10, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b, IL-20R alpha, IL-20R beta, IL-21R, IL-22R alpha 1, IL-27R alpha, IL-2R alpha, IL-31R alpha, IL-36 beta / IL-1F8, IL-3R alpha, IL-5R alpha, IL-6R, Insulin R / CD220, RELT / TNFRSF19L, Siglec-9 / CD329, SPARC / Osteonectin, TGF beta R1 / ALK 5, Tie-2 / TEK, BAFF R / TNFRSF13C, TRANCE / TNFSF11, SCF / KITLG, Angiopoietin-3 / ANGPTL1, Activin C / INHBC, BMPR-IA / ALK-3, PECAM-1 / CD31, BRAK / CXCL14, FGF-10 / KGF-2, GDF-11 / BMP-11, GDF-8 / Myostatin, GFR alpha-1 / GDNF R, ICAM-2 / CD102, IL-13R alpha 1, IL-1R-4 / ST2 / IL-33R, IL-36 gamma / IL-1F9, LIGHT / TNFSF14, MCP-3 / MARC / CCL7, SAA / Serum Amyloid A, Siglec-5 / CD170 / Siglec-F, TMEFF1 / Tomoregulin-1, TNF R2 / TNFRSF1B, WISP-1 / CCN4, IL-1 alpha / IL-1F1, IP-10 / CXCL10, TWEAK / TNFSF12, ALCAM / CD166, CD40 / TNFRSF5, Endoglin / CD105, ErbB4 / HER4, GITR / TNFRSF18, IL-7R alpha / CD127, Lymphotoxin beta R / TNF RIII / TNFRSF3, GASP-1 / WFIKKNRP, IL-36Ra / IL-1F5, IL-1ra / IL-1RN / IL-1 F3 / IRAP, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, FGF-7 / KGF, HGFR / MET, TWEAK R / TNFRSF12, Fas / TNFRSF6 / Apo-1, MIP-3 alpha / CCL20, Pentraxin-3 / TSG-14, 6Ckine / CCL21, Activin R1B / ALK-4, Adiponectin / ACRP30, ALK-6 / BMPR-IB, BMP-15 / GDF-9B, Betacellulin / BTC, MEC / CCL28, CD30 / TNFRSF8, CD30 Ligand / TNFSF8, CD80 / B7-1, Chordin-Like-1 / CHRDL1, Coagulation Factor III / Tissue Factor / Thromboplastin / F3, Cardiotrophin-1 / CT-1, CTACK / CCL27, Crossveinless-2 / CV-2 / BMPER, DcR3 / TNFRSF6B, DR3 / TNFRSF25, DR6 / TNFRSF21, EG-VEGF / PK1, ENA-78 / CXCL5, EN-RAGE / S100 A12, Eotaxin-2 / MPIF-2 / CCL24, Eotaxin-3 / CCL26, FGF R5 / FGFRL1, Fractalkine / CX3CL1, GASP-2 / WFIKKN, GCP-2 / CXCL6, GCSF R / CD114, GITR Ligand / TNFSF18, HCC-1 / CCL14, HCC-4 / CCL16, Hepassocin / HFREP-1, HVEM / TNFRSF14 / CD270, I-309 / TCA-3 / CCL1, ICAM-1 / CD54, ICAM-3 / CD50, IGFBP-rp1 / IGFBP-7, IL-17E / IL-25, IL-18R beta / IL-1R-7 / AcPL / IL-18RAcP, IL-36 alpha / IL-1F6, IL-37 / IL-1F7, IL-36 gamma / IL-1RP2 / IL-1F9, IL-1R-3 / IL-1RAcP, IL-18 R alpha / IL-1R-5, IL-1Rrp2 / IL-1R-6, IL-26 / AK155, IL-28A / IFN lambda 2, IL-29 / IFN-lambda 1, IL-2R beta / CD122, I-TAC / CXCL11, Integrin alpha-M / CD11b / MAC-1, Kininogen / Kininostatin, Lipocalin-2 / NGAL, L-Selectin / CD62L, Lymphotactin / XCL1, MCP-4 / CCL13, MDC / CCL22, MIP-1 delta / CCL15, MIP-3 beta / CCL19, NAP-2 / PPBP / CXCL7, NGFR / TNFRSF16, NOV / CCN3, NRG1-alpha / HRG1-alpha, NRG1-beta 1 / HRG1-beta 1, Osteoprotegerin / TNFRSF11B, Osteoactivin / GPNMB, OX40 Ligand / TNFSF4, PARC / CCL18, Platelet Factor 4 / CXCL4, Pref-1 / DLK-1 / FA1, RANK / TNFRSF11A, SCF R / CD117 / c-kit, gp130 / sgp130, Soggy-1 / DkkL1, Sonic Hedgehog N-Terminal / Shh-N, Siglec-5 / CD170, TACI / TNFRSF13B, TARC / CCL17, TECK / CCL25, TNF beta / TNFSF1 / LT-alpha, BCMA / TNFRSF17, Thrombopoietin / TPO, TRAIL / TNFSF10, TRAIL R1 / TNFRSF10A / DR4, TRAIL R2 / TNFRSF10B / DR5, TRAIL R3 / TNFRSF10C, TRAIL R4 / TNFRSF10D, TROY / TNFRSF19, PD-ECGF / Thymidine Phosphorylase, VCAM-1 / CD106, TCK-1 / CXCL7, IL-2R gamma / Common gamma Chain, IL-22R alpha 2, GDF-5 / BMP-14, IL-1F10 / IL-1HY2 / FIL1 theta, IL-1-RAPL-1, IL-1R-8, IL-1R-9 / IL-1-RAPL-2, IL-8R A / CXC-R1, IL-8R B / CXC-R2, SDF-1 / CXCL12, TGF beta 5, Thymopoietin / LAP2
靶標數	507

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Tyrosine Phosphorylation Array C1 Membranes
Blocking Buffer
Biotinylated Anti-Phosphotyrosine Antibody
1,000X HRP-Streptavidin Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
Incubation Tray w/ Lid
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
User Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or plastic Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system:
- CCD Camera
- X-Ray Film and a suitable film processor
- Gel documentation system
- Or another chemiluminescent detection system capable of imaging a western blot

Protocol Outline