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| 規(guī)格 | 4 樣本、8 樣本 |
| 樣本類型 | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| 原理方法 | Sandwich-based |
| 檢測方法 | Fluorescence Laser Scanner |
| 定量與否 | Semi-Quantitative |
| 載體 | Glass Slide |
| 適用種屬 | Human |
| 檢測靶標(biāo) | EGFR, ErbB3, IGF-1 R, IGFBP-3, 4E-BP1, AKT, bad, ERK1/2, mTOR, p53, PDK1, PTEN, RPS6, RSK1, VEGFR2, VEGFR3, bax, Bcl-2, BID, BIM, Caspase-3, Caspase-8, HSP27, p21, XIAP, p27, ATM, Caspase-7, CHK1, CHK2, JNK, SMAD2, TAK1, MSK1, NBS1, TGF beta 2, ACK1, ALK, AXL, beta-Catenin, BMX, BTK, c-Abl, CD45, CD61, Claudin-3, CXCR4, Cyclin D1, Dtk, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, Epo R, ErbB2, FADD, FAK, FER, FGFR1, FGFR2, FOXO1, FRK, FYN, Hck, HSP90, IFN-alpha/beta R1, IFN-gamma R1, IGF-2 R, IL-2 R alpha, ITK, LCK, LTK, Lyn, M-CSF R, MATK, MuSK, NR3C3, PDGF R alpha, PDGF R beta, PPARg2, PYK2, Ret, ROR1, ROR2, Ryk, SMAD1, SMAD4, SMAD5, SRMS, SYK, TEC, Tie-1, TNK1, TRKB, TXK, TYK2, TYRO10, VEGFR1, ZAP70, BLNK, CAMK1, CAMK4, Caspase-6, CD247, CDC37, HDAC1, HDAC3, HDAC6, PIM1, SP1, TLK1, ARHGEF2, KCNA3, KCNB1, MEF2A, RGS16, SREBF1, Caspase-1, Caspase-2, Caspase-9, CD28, CD4, CD5, CDC25B, CRK, Cyclin E1, DAPP1, DDR1, Fgr, GRIA2, GRK1, ICK, JunB, MDM2, MEF2C, MKK7, NFATC3, Paxillin, PLA2G4A, TrkA, ROS, 14-3-3 beta, 14-3-3 zeta, ASK1, Catalase, CD32, CDK2, Cortactin, CrkL, EPB41, Ephrin-B2, Ezrin, Filamin A, G3BP, KSR1, MARCKS, SHP-1, STAT3, ARAF, ATF1, ATF4, BCR, BRCA1, DAXX, DDX5, DUSP1, E2F1, EIF4B, EIF4G1, GAB2, GJA1, GSK3A, MITF, MLKL, PAK1, PAK3, PEA15, PLCB3, PLCG2, PPP1R1B, PTK6, RACGAP1, RB1, RELB, SYN1, WASF1, ATF2, ATRIP, CD19, EIF4E, HDAC4, HSF1, KCNIP3, PAK4, PRKCB, RHOA, VAV2, CDK7, GRIA1, MAP3K1, MEF2D, NFATC4, RASGRF1, VASP, FRS2, ATR, CDC25A, CDK1, Cyclin B1, LKB1, SHC, SHIP1, SHP-2, JAK1, JAK2, Src, STAT1, STAT6, MEK1, MKK3, MSK2, p38, HDAC2, c-Rel, nfkb2, C-Fos, c-Jun, STAT2, Blk, JAK3, CaMK2D, CBL, CPI17 alpha, CTNND1, Bcl-xL, AMPK beta 1, Androgen R, B-raf, Caveolin-1, CDC25C, ERK2, GRIN2A, GRK2, IkB-beta, IKK alpha, iNOS, IRS1, MEK2, MKK4, MST1, PKC a, PP2A, RAC1, SMC1, STAT5b, Vinculin, WNK1, WWOX, PAK2, PKMYT1, Rictor, STAT4, Amyloid beta 1-40, beta-Arrestin-1, CAMK2A, GAP43, GATA-1, MDM4, PKC-theta, PKC-zeta, PKR, RAD51, STMN1, IL-4R, LAT, LCP2, LSD1, ULK1, Histone H3, JUND, eNOS / NOS3, FoxO3 / FKHRL1, VE-Cadherin / CDH5, IL-10R alpha, IL-3R alpha, Insulin R / CD220, Integrin beta-1, Siglec-2 / CD22, TGF beta R1 / ALK 5, Tie-2 / TEK, PECAM-1 / CD31, IL-13R alpha 1, ErbB4 / HER4, IL-7R alpha / CD127, HGFR / MET, Fas / TNFRSF6 / Apo-1, c-Myc / MYC, Coagulation Factor III / Tissue Factor / Thromboplastin / F3, Cytokeratin 18 / KRT18, Flt-3 / Flk-2, FosB / G0S3, ICAM-1 / CD54, IkB-epsilon / NF-kappa-BIE, IKK-gamma / NEMO / FIP-3, Cytokeratin 8 / KRT8, MKK6 / MEK6, NGFR / TNFRSF16, p130Cas / BCAR1 / CAS, p63 / TP73L / KET, SCF R / CD117 / c-kit, MAPT / Tau, Survivin / BIRC5, Histone Deacetylase 8 / HDAC8, RYR2, AURKA, CREB1, GSK3B, MAP3K8, MAPK6, NF2, PIP5K3, RAF1, RPS6KA3, TOP2A, VAV1, AKT1S1, DOK1, GRIN2B, IRF3, PRKACA, SYT1, ELK1, GRIN1, PLK1, TSC2, CLDN6, CLDN7, PLD1, PLD2, PLCG1, AURKB, AURKC, CDK5, LIMK1, ACETYL-CoA, ADD1, AMPK alpha 1, Beclin1, Casein-Kinase-1a, Casein-Kinase-2b, Cofilin, Cyclin-D2, DAB1, DNM1, EEF-2, EIF2a, ERa, GABRB1, HDAC5, His-H2A.x, HNF-4a, HRS, HSL, IkB alpha, IKK beta, ITG beta 4, LaminA+C, MKNK1, MUCI, MYPT1, NF-KB, P300, P70S6K, PARP, PKC-d, PKC-epsilon, PKD1, PKD2, Smad2/3, SNCA, STAT5, TYH, WASP |
| 靶標(biāo)數(shù) | 393 |
詳細(xì)描述
Protein phosphorylation plays an unusually prominent role in cell signaling, development and growth. The RayBio? G-Series Human Pathway Explorer Phosphorylation Antibody Array 1 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein phosphorylations and be used to monitor the activation or function of important biological pathways.
RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio? Human Pathway Explorer Phosphorylation Antibody Array 1 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 393 different human signaling pathway proteins in cell lysates. By monitoring the changes in protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
To use the RayBio? G-Series Human Pathway Explorer Phosphorylation Antibody Array 1, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phospho Tyr/Thr/Ser antibodies are then used to detect the phosphorylated Tyrosine/Threonine/Serine on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
14-3-3 zeta, 14-3-3b, 4E-BP1, AB 1-40, ABL1, ACETYL CoA, ACK1, ADD1, AKT, AKT1S1, ALK, AMPK a1, AMPK b1, AR, ARAF, ARHGEF2, ASK1, ATF1, ATF2, ATF4, ATM, ATR, ATRIP, AURKA, AURKB, AURKC, AXL, BAD, BAX, BCL-2, BCL-XL, BCR, Beclin1, beta-Arrestin 1, beta-Catenin, BID, BIM, BLK, BLNK, BMX, B-raf, BRCA1, BTK, CAMK1, CAMK2A, CAMK2d, CAMK4, Casein Kinase 1a, Casein Kinase 2b, Caspase 1, Caspase 2, Caspase 3, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Catalase, Caveolin-1, CBL, CD19, CD247, CD28, CD32, CD4, CD45, CD5, CD61, CDC25A, CDC25B, CDC25C, CDC37, CDK1, CDK2, CDK5, CDK7, c-Fos, CHK1, CHK2, c-Jun, Claudin-3, CLDN6, CLDN7, c-Myc, Cofilin, Cortactin, CPI17 alpha, CREB1, c-Rel, CRK, CRKL, CTNND1, CXCR4, Cyclin B1, Cyclin D1, Cyclin D2, Cyclin E1, Cytokeratin 18, Cytokeratin 8, DAB1, DAPP1, DAXX, DDR1, DDX5, DNM1, DOK1, DTK, DUSP1, E2F1, EEF-2, EGFR, EIF2a, EIF4B, EIF4E, EIF4G1, ELK1, eNOS, EPB41, EphA1, EphA2, EphA3, EPHA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, Ephrin B2, EPO R, ERa, ErbB2, ErbB3, ErbB4, ERK1/2, ERK2, Ezrin, Factor 3, FADD, FAK, FAS, FER, FGFR1, FGFR2, FGFR2 (a isoform), FGR, Filamin A, Flt-3, FosB, FoxO1, Foxo3, FRK, FRS2, FYN, G3BP, GAB2, GABRB1, GAP43, GATA-1, GJA1, GRIA1, GRIA2, GRIN1, GRIN2A, Grin2b, GRK1, GRK2, GSK3a, GSk3b, HCK, HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC8, His H2A.x, Histone H3, HNF-4a, HRS, HSF1, HSL, HSP27, HSP90, ICAM-1, ICK, IFN-a/b R1, IFN-gamma R1, IGF-1 R, IGFBP-3, IGF-II R, IkB beta, IkB epsilon, IkBa, IKK gamma, IKKa, IKKb, IL-10 R alpha, IL-13 Ra1, IL-2 sRa, IL-3 R alpha, IL-4 R, IL-7 R alpha, iNOS, Insulin R, IntegrinB1, IRF3, IRS1, ITGB4, ITK, JAK1, JAK2, JAK3, JNK, JunB, JunD, KCNA3, KCNB1, KCNIP3, KSR1, LaminA+C, LAT, LCK, LIMK1, LKB1, LSD1, LTK, Lyn, MAP3K1, MAP3K8, MAPK6, MARCKS, MATK, M-CSF R, MDM2, MDM4, MEF2A, MEF2C, MEF2D, MEK1, MEK2, MET, MITF, MKK3, MKK4, MKK6, MKK7, MKNK1/MNK1, MLKL, MSK1, MSK2, MSP/MST1, mTOR, MUCI, MUSK, MYPT1, Nbs1, NF2, NFATC3, NFATC4, NF-KB (P65), NFkb2, NGFR, NR3C3, P130Cas, P21, P27, P300, P38, P53, P63, P70S6K, PAK1, PAK2, PAK3, PAK4, PARP, Paxillin, PDGF Ra, PDGF Rb, PDK1, PEA15, PECAM-1, PIM1, PIP5K3, PKC a, PKC d, PKC epsilon, PKC theta, PKC zeta, PKD1, PKD2, PKMYT1, PKR, PLA2G4A, PLCB3, PLCg1, PLCG2, PLD1, PLD2, PLK1, PP2A, PPARg2, PPP1R1B, PRKACA, PRKCB, PTEN, PTK6, PYK2, Rac1, RACGAP1, RAD51, Raf1, RASGRF1, RB1, RELB, Ret, RGS16, RhoA, Rictor, ROR1, ROR2, ROS, RPS6, RPS6KA3, RSK1, RYK, RYR2, SCF R, SHC, SHIP1, SHP-1, SHP-2, Siglec-2, SLP-76/LCP2, Smad1, Smad2, Smad2/3, smad4, Smad5, SMC1, SNCA, SP1, SRC, SREBF1, SRMS, STAT1, STAT2, STAT3, STAT4, STAT5, STAT5b, Stat6, STMN1, Survivin, SYK, SYN1, SYT1, TAK1, Tau, Tec, TGF-B2, TGF-beta RI, Tie-1, Tie-2, TLK1, TNK1, TOP2A, TrkA, TrkB, Tuberin / TSC2, TXK, TYH, TYK2, TYRO10 (DDR2), ULK1, VASP, VAV1, VAV2, VE-Cadherin, VEGF-R1, VEGF-R2, VEGFR3, Vinculin, WASF1, WASP, WNK1, WWOX, XIAP, ZAP70
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