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規(guī)格

規(guī)格	8 樣本、4 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Label-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標(biāo)	Cystatin C, Resistin, Trappin-2, ACE-2, PAI-1, Albumin, Clusterin, FABP1, AMPKa1, p53, Caspase-3, Caspase-8, cIAP-2, Cytochrome C, HSP27, HSP60, HSP70, HTRA2, IGFBP-5, Livin, p21, SMAC, XIAP, p27, A1BG, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, ADAMTSL-2, Afamin, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha Lactalbumin, Alpha-fetoprotein, ALPP, AMICA, Amylin, Annexin A7, APC, APCS, Apelin, ASPH, Attractin, B3GNT1, BAF57, BCAM, Beta Defensin 4, beta IG-H3, Biglycan, BMP-9, BMX, BNIP2, BNP, BTK, c-Abl, C-Peptide, C3a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin D, Calcitonin, Calreticulin, CART, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD38, CD45, CD46, CD47, CD59, CD61, CD71, CD74, CD79 alpha, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chymase, CK-MB, Claudin-3, Claudin-4, COCO, Complement Factor H, Contactin-1, CPN2, Creatinine, CRTAM, CSH1, CutA, Cyclin D1, Cystatin A, Cystatin B, D-Dimer, DBI, Desmin, DLL1, DLL4, DMP-1, E-Cadherin, Endothelin Receptor A, Enolase 2, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP2, FABP3, FAK, FAP, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FOXO1, FoxP3, FRK, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas 1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, Hck, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP40, HSP90, HSPA8, IBSP, IL-34, IMP1, INSL3, INSRR, Integrin alpha-5, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, LOX-1, LPS, LRG1, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, Neprilysin, Nestin, NET1, Netrin G2, Netrin-1, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Omentin, Osteocalcin, P-Cadherin, PAK7, Pancreastatin, PCAF, PD-1, PGRP-S, PI 16, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, Procalcitonin, Progesterone, Prohibitin, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, PSP, PTH, PTHLP, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Ret, RIP1, ROCK1, ROCK2, ROR1, ROR2, Ryk, S100 A6, S100 A8, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A12, Serpin A4, Serpin A9, Serpin B5, Serpin D1, Serpin G1, Serpin I1, SERTAD2, SHBG, SNCG, SOST, SOX17, SOX2, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, SYK, Syndecan-1, Syndecan-3, TAF4, TEC, TFF1, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, TRKB, Troponin C, Troponin I, Troponin T, TRPC1, TRPC6, TRPM7, TSH, TSLP, TXK, TYK2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VGF, VIP Receptor 2, Vitamin D Receptor, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, ZAG, ZAP70, 11b-HSD1, Alpha 1 AG, Alpha 1 Microglobulin, APN, APP, ARB1, BAI-1, Beta 2M, C1qTNF1, C2, C5/C5a, C7, C8b, C9, CBG, Chromogranin A, CLEC14A, Cytokeratin18, Cytokeratin19, DEFA1/3, Endorphin Beta, ENPP2, Factor XIII B, Fc gamma RIIIB, Fcg RIIB/C, GRP75, hCGb, IDUA, IL-28B, LTF, Nesfatin, OCT3/4, Pappalysin-1, PEPSINOGEN I, PEPSINOGEN II, PI 3Kinase p85 alpha, pro-Glucagon, ROS, S-100b, S100A4, VEGF R1, Vitamin D-BP, SPARC-Like 1, Cathepsin B / Pro-Cathepsin B, IL-33 / IL-1F11, Angiopoietin-like protein 3, Angiopoietin-like protein 4, Apolipoprotein A-I / ApoA1, Apolipoprotein A-II / ApoA2, Apolipoprotein A-IV / ApoA4, Apolipoprotein B / ApoB, Apolipoprotein B-100 / Apo B-100, Apolipoprotein C-I / ApoC1, Apolipoprotein C-II / ApoC2, Apolipoprotein C-III / ApoC3, Apolipoprotein D / ApoD, Apolipoprotein E / ApoE, Apolipoprotein E-III / ApoE3, Apolipoprotein H / ApoH, BLAME / SLAMF8, CK beta 8-1 / CCL23, Coagulation Factor II / Thrombin / F2, HSP32 / HO-1 / HMOX1, PDX-1 / IPF1, CD90 / Thy1, Adipsin / Complement Factor D, Angiotensinogen / Serpin A8, CD44 / Heparan Sulfate Proteoglycan, CLEC3B / Tetranectin, Carnosine Dipeptidase-1 / CNDP1, Contactin-2 / TAG1, SCGF / CLEC11a, CD36 / SR-B3, TROP1 / EpCAM, Serpin A1 / Alpha-1-Antitrypsin, Serpin F1 / PEDF, 2B4 / CD244, ACE / CD143, APE1 / APEX1, Carboxypeptidase A1 / CPA1, CD26 / DPPIV, CD55 / DAF, Calsyntenin-1 / Alcadein-alpha, COX-2 / PTGS2, CRP / C-Reactive Protein, DCBLD2 / ESDN, FABP4 / A-FABP, HE4 / WFDC2, Cytokeratin 8 / KRT8, Osteopontin / SPP1, OX40 / TNFRSF4, Park7 / DJ-1, Pancreatic polypeptide / ProPPY / PP, Pleiotrophin / PTN, Serpin A3 / Alpha-1-Antichymotrypsin, Serpin A5 / Protein C Inhibitor, ADAM-17 / TACE, TFF3 / Trefoil Factor 3, TIM-1 / KIM-1, 4-1BB / TNFRSF9 / CD137, Trypsin 1 / PRSS1, Visfatin / PBEF1, ADAMTS-1 / METH1, Alpha-2 Microglobulin, BAFF / TNFSF13B, Survivin / BIRC5, Apolipoprotein M / ApoM
靶標(biāo)數(shù)	493

詳細(xì)描述

Introduction

Protein Oxidation has been linked to aging, cancer and other diseases. RayBio? Human Protein Oxidation Antibody Array 2 is specifically designed for simultaneous identification of the relative levels of oxidation of 493 different human proteins in cell lysate, culture supernatant, serum, plasma and other biological samples. By monitoring the changes in protein oxidation in your experimental model system, you can verify oxidative damage without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.

RayBio? Human Protein Oxidation Antibody Arrays utilizes the ‘biotin probes’ method to biotin label carbonyl groups of oxidized proteins. In this method, the biotin probes first react with carbonyl groups of oxidized proteins to form the unstable Schiff bases, which are then further reduced to more stable amines. The biotin labeled sample then is added into antibody array glass slide wells. The antibody array slide wells are washed. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

Product Features

High sensitivity and specificity
Low sample volume (10-100 μl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

11b-HSD1, 2B4, 4-1BB, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adipsin, Afamin, AFP, ALBUMIN, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, ASPH, Attractin, B3GNT1, BAF57, BAFF, BAI-1, BCAM, Beta 2M, Beta Defensin 4, Beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, Btk, C2, C3a, C5/C5a, C7, C8B, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC3B, Clusterin, CNDP1, COCO, Complement factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-peptide, CPN2, Creatinine, CRP, CRTAM, CSH1, cTnT / Troponin T, CutA, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, DBI, DCBLD2, D-Dimer, DEFA1/3, Defensin, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endorphin Beta, Endothelin Receptor A, Enolase 2, ENPP2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII A, Factor XIII B, FAK, FAP, Fc RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FoxO1, FoxP3, FRK, FSH, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, hCGb, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, IBSP, IGF2BP1, IGFBP-5, IL-23p19, IL-33, IL-34, IL36RN, INSL3, INSRR, Integrin alpha V, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, Livin, LOX-1, LPS, LRG1, LTF, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, NEP, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Ntn1, OCT3/4, Omentin, Osteocalcin, Osteopontin, OX40, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARK7, P-Cadherin, PCAF, PD-1, PDX-1, PEDF , PEPSINOGEN I, PEPSINOGEN II, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Prohibitin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Resistin, RET, RIP1, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100A4, S100A6, S100A8, S-100b, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin I1, SERTAD2, SHBG, SMAC, SNCG, Somatotropin, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, Tec, TFF1, TFF3, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TIM-1, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, Trappin-2, TRKB, Troponin C, Troponin I, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, Tyk2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VEGF R1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, XIAP, ZAG, ZAP70

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components