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規(guī)格

規(guī)格	2 樣本、4 樣本、8 樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測(cè)方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測(cè)靶標(biāo)	IFN-gamma, IL-10, IL-13, IL-15, IL-2, IL-4, LIF, EGFR, GM-CSF, IL-12 p40, IL-6, PDGF-BB, IL-5, Growth Hormone, IL-11, IL-12 p70, Leptin, PDGF-AA, EGF, GCSF, p21, IL-23 p19, IL-7, IL-9, PDGF R beta, Prolactin, TSLP, IL-10R beta, IL-13R alpha 2, IL-21R, IL-2R alpha, IL-6R, IL-13R alpha 1, IL-28A / IFN lambda 2, IL-29 / IFN-lambda 1, IL-2R beta / CD122, gp130 / sgp130, Thrombopoietin / TPO, IL-2R gamma / Common gamma Chain
靶標(biāo)數(shù)	39

詳細(xì)描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGF Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	EGFR Gene Symbol: IL23A SGRF UNQ2498/PRO5798 Gene ID: 51561 Accession #: Q9NPF7 ELISA Kit: RayBio Human IL-23 ELISA	GCSF Gene Symbol: IL13RA2 IL13R Gene ID: 3598 Accession #: Q14627 ELISA Kit: RayBio Human IL-13 Ra2 ELISA	GM-CSF Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	gp130 (sgp130) Gene Symbol: PRL Gene ID: 5617 Accession #: P01236 ELISA Kit: RayBio
Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	IFN-gamma Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-10 Gene Symbol: IL12B NKSF2 Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-10 R beta Gene Symbol: CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 Gene ID: 1026 Accession #: P38936 ELISA Kit: RayBio	IL-11 Gene Symbol: IL2RB Gene ID: 3560 Accession #: P14784 ELISA Kit: RayBio Human IL-2 R beta ELISA
IL-12 p40 Gene Symbol: IL10RB CRFB4 D21S58 D21S66 Gene ID: 3588 Accession #: Q08334 ELISA Kit: RayBio Human IL-10 R beta ELISA	IL-12 p70 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-13 Gene Symbol: IFNL1 IL29 ZCYTO21 Gene ID: 282618 Accession #: Q8IU54 ELISA Kit: RayBio Human IL-29 ELISA	IL-13 R alpha 1 Gene Symbol: THPO MGDF Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	IL-13 R alpha 2 Gene Symbol: TSLP Gene ID: 85480 Accession #: Q969D9 ELISA Kit: RayBio Human TSLP ELISA
IL-15 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-2 Gene Symbol: IL13 NC30 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-2 R alpha Gene Symbol: PDGFA PDGF1 Gene ID: 5154 Accession #: P04085 ELISA Kit: RayBio Human PDGF-AA ELISA	IL-2 R beta (CD122) Gene Symbol: PDGFB PDGF2 SIS Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	IL-2 R gamma (Common gamma Chain) Gene Symbol: LEP OB OBS Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA
IL-21 R Gene Symbol: PDGFRB PDGFR PDGFR1 Gene ID: 5159 Accession #: P09619 ELISA Kit: RayBio Human PDGF R beta ELISA	IL-23 p19 Gene Symbol: EGFR ERBB ERBB1 HER1 Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-29 (IFN-lambda 1) Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-4 Gene Symbol: IL13RA1 IL13R IL13RA Gene ID: 3597 Accession #: P78552 ELISA Kit: RayBio Human IL-13 R alpha 1/IL-13 R1 ELISA
IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	IL-6 R Gene Symbol: IL2RG Gene ID: 3561 Accession #: P31785 ELISA Kit: RayBio	IL-7 Gene Symbol: IL21R NILR UNQ3121/PRO10273 Gene ID: 50615 Accession #: Q9HBE5 ELISA Kit: RayBio Human IL-21 R ELISA	IL-9 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA
Leptin Gene Symbol: CSF2 GMCSF Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	LIF Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	p21 Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	PDGF R beta Gene Symbol: LIF HILDA Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA	PDGF-AA Gene Symbol: IFNL2 IL28A ZCYTO20 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA
PDGF-BB Gene Symbol: IL6ST Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	Prolactin Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	Thrombopoietin (TPO) Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha ELISA	TSLP Gene Symbol: IL9 Gene ID: 3578 Accession #: P15248 ELISA Kit: RayBio Human IL-9 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human JAK/STAT Pathway Antibody Array C2 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AA-PRELIM.pdf

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