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規格

規格	4樣本(產品加服務)、2樣本、4樣本、8樣本、2樣本(產品加服務)、8樣本(產品加服務)
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標	Cystatin C, Albumin, Clusterin, AMICA, Angiopoietin-4, CD138, CD163, CRTAM, DLL1, Fetuin A, FOLR1, HAI-2, IL-27, LAG-3, LDL R, Pepsinogen 1, RBP4, SOST, Syndecan-1, TFPI, Thrombospondin-1, Troponin I, uPA, IL-17RB, aFGF / FGF-1, TRANCE / TNFSF11, BRAK / CXCL14, WISP-1 / CCN4, GASP-1 / WFIKKNRP, GASP-2 / WFIKKN, GCSF R / CD114, TACI / TNFRSF13B, BAFF / TNFSF13B
靶標數	32

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
aFGF (FGF-1) Gene Symbol: FGF1 Gene ID: 2246 Accession #: P05230 ELISA Kit: RayBio Human aFGF ELISA	Albumin Gene Symbol: ALB Gene ID: 213 Accession #: P02768 ELISA Kit: RayBio Human Albumin ELISA	AMICA Gene Symbol: AMICA1 Gene ID: 120425 Accession #: Q86YT9 ELISA Kit: RayBio Human AMICA ELISA	Angiopoietin-4 Gene Symbol: ANGPT4 Gene ID: 51378 Accession #: Q9Y264 ELISA Kit: RayBio Human ANGPT4 ELISA	BAFF Gene Symbol: TNFSF13B Gene ID: 10673 Accession #: Q9Y275
CD163 Gene Symbol: CD163 Gene ID: 9332 Accession #: Q86VB7 ELISA Kit: RayBio Human CD163 ELISA	Clusterin Gene Symbol: CLU Gene ID: 1191 Accession #: P10909 ELISA Kit: RayBio Human Clusterin ELISA	CRTAM Gene Symbol: CRTAM Gene ID: 56253 Accession #: O95727 ELISA Kit: RayBio Human CRTAM ELISA	CXCL14 (BRAK) Gene Symbol: CXCL14 Gene ID: 9547 Accession #: O95715 ELISA Kit: RayBio Human CXCL14 ELISA	Cystatin C Gene Symbol: CST3 Gene ID: 1471 Accession #: P01034 ELISA Kit: RayBio Human Cystatin C ELISA
DLL1 Gene Symbol: DLL1 Gene ID: 28514 Accession #: O00548 ELISA Kit: RayBio Human DLL1 ELISA	Fetuin A Gene Symbol: AHSG Gene ID: 197 Accession #: P02765 ELISA Kit: RayBio Human Fetuin A ELISA	FOLR1 Gene Symbol: FOLR1 Gene ID: 2348 Accession #: P15328 ELISA Kit: RayBio Human FOLR1 ELISA	GASP-1 (WFIKKNRP) Gene Symbol: GPRASP1 Gene ID: 9737 Accession #: Q5JY77 ELISA Kit: RayBio Human GASP-1 ELISA	GASP-2 (WFIKKN) Gene Symbol: GPRASP2 Gene ID: 114928 Accession #: Q96D09 ELISA Kit: RayBio Human GASP-2 ELISA
GCSF R (CD114) Gene Symbol: CSF3R Gene ID: 1441 Accession #: Q99062 ELISA Kit: RayBio Human G-CSF R ELISA	HAI-2 Gene Symbol: SPINT2 Gene ID: 10653 Accession #: O43291 ELISA Kit: RayBio Human HAI-2 ELISA	IL-17 RB Gene Symbol: IL17RB Gene ID: 55540 Accession #: Q9NRM6 ELISA Kit: RayBio Human IL-17B R ELISA	IL-27 Gene Symbol: IL27 Gene ID: 246778 Accession #: Q8NEV9	LAG-3 Gene Symbol: LAG3 Gene ID: 3902 Accession #: P18627 ELISA Kit: RayBio Human LAG3 ELISA
LDL R Gene Symbol: LDLR Gene ID: 3949 Accession #: P01130 ELISA Kit: RayBio Human LDL R ELISA	Pepsinogen 1 Gene Symbol: PGA3/PGA4/PGA5 ELISA Kit: RayBio Human Pepsinogen I ELISA	RBP4 Gene Symbol: RBP4 Gene ID: 5950 Accession #: P02753 ELISA Kit: RayBio Human RBP-4 ELISA	SOST Gene Symbol: SOST Gene ID: 50964 Accession #: Q9BQB4 ELISA Kit: RayBio Human SOST ELISA	Syndecan-1 Gene Symbol: SDC1 Gene ID: 6382 Accession #: P18827 ELISA Kit: RayBio Human Syndecan 1 ELISA
TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Gene ID: 23495 Accession #: O14836 ELISA Kit: RayBio Human TACI ELISA	TFPI Gene Symbol: TFPI Gene ID: 7035 Accession #: P10646 ELISA Kit: RayBio Human TFPI ELISA	Thrombospondin 1 Gene Symbol: THBS1 Gene ID: 7057 Accession #: P07996 ELISA Kit: RayBio Human Thrombospondin 1 ELISA	TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: 8600 Accession #: O14788	Troponin I Gene Symbol: TNNI2 Gene ID: 7136 Accession #: P48788 ELISA Kit: RayBio Human Troponin I ELISA
uPA Gene Symbol: PRAP1 Gene ID: 118471 Accession #: Q96NZ9 ELISA Kit: RayBio Human uPA ELISA	WISP-1 (CCN4) Gene Symbol: WISP1 Gene ID: 8840 Accession #: O95388 ELISA Kit: RayBio Human WISP-1 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C12 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAH-CYT-12.pdf

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