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規(guī)格

規(guī)格	8樣本、2樣本(產(chǎn)品加服務(wù))、4樣本(產(chǎn)品加服務(wù))、8樣本(產(chǎn)品加服務(wù))、4樣本、2樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標(biāo)	Contactin-1, E-Cadherin, ESAM, PD-1, Syndecan-1, Syndecan-3, Cadherin-4, CD28, CD6, CD99, ICOS, Nectin-1, Syndecan-4, CTLA-4 / CD152, CD40 Ligand / TNFSF5, CD58 / LFA-3, B7-H3 / CD276, Contactin-2 / TAG1, CD155 / PVR, CD40 / TNFRSF5, JAM-A / CD321 / F11R, B7-H1 / CD274 / PD-L1, B7-H2 / ICOS Ligand, CD80 / B7-1, DNAM-1 / CD226, JAM-B / VE-JAM / CD322
靶標(biāo)數(shù)	26

詳細(xì)描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
B7-H1 (CD274/PD-L1) Gene Symbol: CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 Gene ID: 29126 Accession #: Q9NZQ7 ELISA Kit: RayBio Human B7H1/PD-L1 ELISA	B7-H2 (ICOS Ligand) Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653 Gene ID: 102723996 23308 Accession #: O75144 ELISA Kit: RayBio Human B7-H2/ICOS Ligand ELISA	B7-H3 (CD276) Gene Symbol: CD276 B7H3 PSEC0249 UNQ309/PRO352 Gene ID: 80381 Accession #: Q5ZPR3 ELISA Kit: RayBio Human B7-H3/CD276 ELISA	Cadherin-4 Gene Symbol: CDH4 Gene ID: 1002 Accession #: P55283 ELISA Kit: RayBio Human Cadherin-4 ELISA	CD6 Gene Symbol: CD6 Gene ID: 923 Accession #: P30203 ELISA Kit: RayBio Human Cluster of Differentiation 6/CD6 ELISA
CD28 Gene Symbol: CD28 Gene ID: 940 Accession #: P10747 ELISA Kit: RayBio Human CD28 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 TNFRSF5 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG CD40L TNFSF5 TRAP Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CD58 (LFA-3) Gene Symbol: CD58 LFA3 Gene ID: 965 Accession #: P19256 ELISA Kit: RayBio Human CD58 ELISA	CD80 (B7-1) Gene Symbol: CD80 CD28LG CD28LG1 LAB7 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80/B7-1 ELISA
CD99 Gene Symbol: CD99 MIC2 MIC2X MIC2Y Gene ID: 4267 Accession #: P14209 ELISA Kit: RayBio Human CD99 ELISA	CD155 (PVR) Gene Symbol: PVR PVS Gene ID: 5817 Accession #: P15151 ELISA Kit: RayBio Human CD155/PVR ELISA	Contactin-1 Gene Symbol: CNTN1 Gene ID: 1272 Accession #: Q12860 ELISA Kit: RayBio Human CNTN1 (Contactin-1) ELISA	Contactin-2 (TAG1) Gene Symbol: CNTN2 AXT TAG1 TAX1 Gene ID: 6900 Accession #: Q02246 ELISA Kit: RayBio Human Contactin-2 ELISA	CTLA-4 (CD152) Gene Symbol: CTLA4 CD152 Gene ID: 1493 Accession #: P16410 ELISA Kit: RayBio
DNAM-1 (CD226) Gene Symbol: CD226 DNAM1 Gene ID: 10666 Accession #: Q15762 ELISA Kit: RayBio Human DNAM-1 ELISA	E-Cadherin Gene Symbol: CDH1 CDHE UVO Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio	ESAM Gene Symbol: ESAM UNQ220/PRO246 Gene ID: 90952 Accession #: Q96AP7 ELISA Kit: RayBio Human ESAM ELISA	ICOS Gene Symbol: ICOS AILIM Gene ID: 29851 Accession #: Q9Y6W8 ELISA Kit: RayBio Human ICOS ELISA	JAM-A (CD321/F11R) Gene Symbol: F11R JAM1 JCAM UNQ264/PRO301 Gene ID: 50848 Accession #: Q9Y624 ELISA Kit: RayBio Human JAM-A/CD321/F11R ELISA
JAM-B (VE-JAM/CD322) Gene Symbol: JAM2 C21orf43 VEJAM UNQ219/PRO245 Gene ID: 58494 Accession #: P57087 ELISA Kit: RayBio Human JAM-B ELISA	Nectin-1 Gene Symbol: PVRL1 HVEC PRR1 Gene ID: 5818 Accession #: Q15223 ELISA Kit: RayBio Human Nectin-1 ELISA	PD-1 Gene Symbol: PDCD1 PD1 Gene ID: 5133 Accession #: Q15116 ELISA Kit: RayBio Human PD-1 ELISA	Syndecan-1 Gene Symbol: SDC1 SDC Gene ID: 6382 Accession #: P18827 ELISA Kit: RayBio Human Syndecan 1 ELISA	Syndecan-3 Gene Symbol: SDC3 KIAA0468 Gene ID: 9672 Accession #: O75056 ELISA Kit: RayBio Human Syndecan-3 ELISA
Syndecan-4 Gene Symbol: SDC4 Gene ID: 6385 Accession #: P31431 ELISA Kit: RayBio Human Syndecan-4 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cell Adhesion Molecule Antibody Array C2 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AA-PRELIM.pdf

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