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規格

規格	8樣本、2樣本、8樣本(產品加服務)、4樣本(產品加服務)、2樣本(產品加服務)、4樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Sandwich-based
檢測方法	Chemiluminescence
定量與否	Semi-Quantitative
載體	Membrane
適用種屬	Human
檢測靶標	BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-9, BMPR-II, COCO, Follistatin, BMPR-IA / ALK-3, GDF-11 / BMP-11, GDF-8 / Myostatin, ALK-6 / BMPR-IB, BAMBI / NMA, Crossveinless-2 / CV-2 / BMPER
靶標數	16

詳細描述

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BAMBI (NMA) Gene Symbol: BAMBI NMA Gene ID: 25805 Accession #: Q13145 ELISA Kit: RayBio Human BAMBI ELISA	BMP-2 Gene Symbol: BMP2 BMP2A Gene ID: 650 Accession #: P12643 ELISA Kit: RayBio Human BMP-2 ELISA	BMP-4 Gene Symbol: BMP4 BMP2B DVR4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA	BMP-6 Gene Symbol: BMP6 VGR Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA
BMP-7 Gene Symbol: BMP7 OP1 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	BMP-8 Gene Symbol: BMP8A Gene ID: 353500 Accession #: Q7Z5Y6 ELISA Kit: RayBio Human BMP-8 ELISA	BMP-9 Gene Symbol: GDF2 BMP9 Gene ID: 2658 Accession #: Q9UK05 ELISA Kit: RayBio Human BMP-9 ELISA	BMPR-IA (ALK-3) Gene Symbol: BMPR1A ACVRLK3 ALK3 Gene ID: 657 Accession #: P36894 ELISA Kit: RayBio Human BMPR-IA ELISA	ALK-6 (BMPR-IB) Gene Symbol: BMPR1B Gene ID: 658 Accession #: O00238 ELISA Kit: RayBio Human ALK-6 ELISA
BMPR-II Gene Symbol: BMPR2 PPH1 Gene ID: 659 Accession #: Q13873 ELISA Kit: RayBio Human BMPR-II ELISA	COCO Gene Symbol: DAND5 CER2 CKTSF1B3 GREM3 SP1 Gene ID: 199699 Accession #: Q8N907 ELISA Kit: RayBio Human COCO ELISA	Crossveinless-2 (CV-2/BMPER) Gene Symbol: BMPER KIAA1965 Gene ID: 168667 Accession #: Q8N8U9 ELISA Kit: RayBio Human Crossveinless-2 ELISA	Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	GDF-11 (BMP-11) Gene Symbol: GDF11 BMP11 Gene ID: 10220 Accession #: O95390 ELISA Kit: RayBio Human GDF-11/BMP-11 ELISA
GDF-8 (Myostatin) Gene Symbol: MSTN GDF8 Gene ID: 2660 Accession #: O14793 ELISA Kit: RayBio Human GDF-8/Myostatin ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human BMP Related Array 1
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^? or EpiChem II Benchtop Darkroom or GE's ImageQuant^? LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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文件

AAH-BMP-1.pdf

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