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規(guī)格

規(guī)格	4樣本、8樣本
樣本類型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
原理方法	Label-based
檢測方法	Fluorescence Laser Scanner
定量與否	Semi-Quantitative
載體	Glass Slide
適用種屬	Human
檢測靶標	TNF alpha, IFN-beta, IL-18, IL-12 p40, IL-6, Bcl-2, Caspase-8, cIAP-2, XIAP, IkB-alpha, ATG12, ATG5, GABARAP, CD14, FADD, LBP, NAIP, TLR1, TLR2, TLR3, TLR4, TRPM7, TYK2, AIM2, BCL2L1, Caspase-5, Cathepsin K, GABARAPL2, GBP2, IFN alpha 5, IFNA2, MAPK1, MAPK8, PIK3CA, RELA, GABARAPL1, OAS1, PLCB2, Caspase-1, JUN, MyD88, NFKB1, RNF31, VDAC2, 14-3-3 epsilon, DEFA6, VDAC1, CARD9, CHUK, FOS, IKK-B, IRAK1, MAP2K3, MAP2K4, MAP3K7IP1, MAPK11, NOD1, OAS2, PIK3CB, PIK3CD, PIK3R2, PLCB1, PLCB3, RIPK2, RIPK3, TBK1, TICAM2, TLR5, TRAF5, TRAF6, VISA, Erbin, IFNA4, IRAK4, MAP2K2, MAPK14, PKN2, PLCB4, RHOA, Caspase-4, NLRC4, RBCK1, CARD18, CYBA, GPRC6A, RNASEL, IL-12 p40/p70, PI3-Kinase p55-gamma, JAK1, STAT1, IRF5, STING, STAT2, IFNAR1, CCL3L1, Akt1, Akt2, PIK3R1, RAC1, RIPK1, Tollip, cAMP, IFNA10, IFNA8, MAPK13, MAP2K7, MAPK12, AKT3, MAP2K1, TXN, IFN-alpha / beta R2, Cathepsin B / Pro-Cathepsin B, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, IL-1 beta / IL-1F2, MIG / CXCL9, MIP-1 beta / CCL4, Toll-like receptor 6 / CD286, CXCL2 / CINC-3 / MIP2, CXCL3 / CINC-2 / MIP2 alpha/beta, CD86 / B7-2, IP-10 / CXCL10, CD40 / TNFRSF5, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, CD80 / B7-1, cIAP-1 / BIRC2 / hIAP-2, CMG-2 / ANTXR2, DEFB-3 / Beta-defensin 103 / BD-3, IKK-gamma / NEMO / FIP-3, I-TAC / CXCL11, MD-2 / LY96, Osteopontin / SPP1, Visfatin / PBEF1, ASC / PYCARD, IKB-b / NFKBIB, GSDMD, NOD2, ANTXR1, MAP3K8, MAPK9, MFN2, P2RX7, PKN1, SHARPIN, TANK, TLR9, TRAF2, IRF3, MAPK3, NLRP1, NLRP3, NLRP6, PRKCD, TLR8, TNFAIP3, TXN2, DEFA3, DEFA4, HSP90AA1, IFNA14, IFNA16, IFNA21, MAP3K7, IFNA17, IFNA6, IFNA7, DEFA5, MFN1, PANX1, TAB2, LAG-1 / CCL4L1, TRAF3, HSP90AB1, CASR, DEFA1, DEFB4A, TP53BP1, TXNIP
靶標數(shù)	181

詳細描述

Product Features

High density arrays
Unbiased detection through direct biotin labeling of samples
Low sample consumption
High detection sensitivity
Same day result
Affordable and simple to use

Target Names

14-3-3 epsilon, AIM2, Akt1, Akt2, AKT3, ANTXR1, ASC / PYCARD, ATG12, ATG5, Bcl-2, BCL2L1, cAMP, CARD18, CARD9, Caspase-1, Caspase-4, Caspase-5, Caspase-8, CASR, Cathepsin B / Pro-Cathepsin B, Cathepsin K, CCL3L1, CD14, CD40 / TNFRSF5, CD80 / B7-1, CD86 / B7-2, CHUK, cIAP-1 / BIRC2 / hIAP-2, cIAP-2, CMG-2 / ANTXR2, CXCL1 / GRO alpha / KC / CINC-1, CXCL2 / CINC-3 / MIP2, CXCL3 / CINC-2 / MIP2 alpha/beta, CYBA, DEFA1, DEFA3, DEFA4, DEFA5, DEFA6, DEFB-3 / Beta-defensin 103 / BD-3, DEFB4A, Erbin, FADD, FOS, GABARAP, GABARAPL1, GABARAPL2, GBP2, GPRC6A, GSDMD, HSP90AA1, HSP90AB1, IFN alpha 5, IFNA10, IFNA14, IFNA16, IFNA17, IFNA2, IFNA21, IFNA4, IFNA6, IFNA7, IFNA8, IFN-alpha / beta R2, IFNAR1, IFN-beta, IkB-alpha, IKB-b / NFKBIB, IKK-B, IKK-E / IKBKE, IKK-gamma / NEMO / FIP-3, IL-1 beta / IL-1F2, IL-12 p40, IL-12 p40/p70, IL-18, IL-6, IL-8 / CXCL8, IP-10 / CXCL10, IRAK1, IRAK4, IRF3, IRF5, I-TAC / CXCL11, JAK1, JUN, LAG-1 / CCL4L1, LBP, MAP2K1, MAP2K2, MAP2K3, MAP2K4, MAP2K7, MAP3K7, MAP3K7IP1, MAP3K8, MAPK1, MAPK11, MAPK12, MAPK13, MAPK14, MAPK3, MAPK8, MAPK9, MCP-1 / CCL2, MD-2 / LY96, MFN1, MFN2, MIG / CXCL9, MIP-1 alpha / CCL3, MIP-1 beta / CCL4, MyD88, NAIP, NFKB1, NLRC4, NLRP1, NLRP3, NLRP6, NOD1, NOD2, OAS1, OAS2, Osteopontin / SPP1, P2RX7, PANX1, PI3-Kinase p55-gamma, PIK3CA, PIK3CB, PIK3CD, PIK3R1, PIK3R2, PKN1, PKN2, PLCB1, PLCB2, PLCB3, PLCB4, PRKCD, RAC1, RANTES / CCL5, RBCK1, RelA, RHOA, RIPK1, RIPK2, RIPK3, RNASEL, RNF31, SHARPIN, STAT1, STAT2, STING, TAB2, TANK, TBK1, TICAM2, TLR1, TLR2, TLR3, TLR4, TLR5, TLR8, TLR9, TNF alpha, TNFAIP3, Tollip, Toll-like receptor 6 / CD286, TP53BP1, TRAF2, TRAF3, TRAF5, TRAF6, TRPM7, TXN, TXN2, TXNIP, Tyk2, VDAC1, VDAC2, VISA, Visfatin / PBEF1, XIAP

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing

Kit Components

RayBio^? L-Series Antibody Array Glass Slide(s)
Spin Columns
Labeling Reagent
Stop Solution
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Streptavidin-Conjugated Fluor
Adhesive Plastic Strips
Labeling Buffer
Lysis Buffer
30 ml Centrifuge Tube

Other Materials Required

Distilled or de-ionized water
Small plastic or glass containers
Orbital shaker or oscillating rocker
1 ml tube
Pipettors, pipette tips and other common lab consumables
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Purify Samples with Spin Column
Biotinylate Samples
Purify biotinylated samples using spin column
Dry the glass slide
Block array surface
Incubate with Samples
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.