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規格

規格	192 tests、48 tests、96 tests、480 tests
樣本類型	Cell Culture Supernatants, Serum
原理方法	Sandwich-based
檢測方法	Flow Cytometry
定量與否	Quantitative
載體	Fluorescent Microbeads
適用種屬	Mouse
檢測靶標	Common beta Chain / GM-CSF R beta / CSF2RB / GM-CSF, IFN-beta / IFNB1, CXCL1 / GRO alpha / KC / CINC-1, RANTES / CCL5, MCP-1 / CCL2, IP-10 / CXCL10, IL-6 / IL6, IL-12 p70 / IL12A, TNF alpha / TNFA, IFN-gamma / IFNG, IL-10 / IL10

詳細描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 μl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GM-CSF Gene Symbol: Csf2rb Aic2b Csf2rb1 Il3rb1 Gene ID: 12983 Accession #: P26955 ELISA Kit: Mouse GM-CSF ELISA Kit	IFN-β Gene Symbol: Ifnb1 Ifb Ifnb Gene ID: 15977 Accession #: P01575 ELISA Kit: Mouse IFN-beta ELISA Kit	KC Gene Symbol: Cxcl1 Gro Gro1 Mgsa Scyb1 Gene ID: 14825 Accession #: P12850 ELISA Kit: Mouse KC ELISA Kit	Rantes Gene Symbol: Ccl5 Scya5 Gene ID: 20304 Accession #: P30882 ELISA Kit: Mouse RANTES ELISA Kit	MCP-1 Gene Symbol: Ccl2 Je Mcp1 Scya2 Gene ID: 20296 Accession #: P10148 ELISA Kit: Mouse MCP-1 ELISA Kit
IP-10 Gene Symbol: Cxcl10 Crg2 Ifi10 Inp10 Scyb10 Gene ID: 15945 Accession #: P17515 ELISA Kit: Mouse CRG-2 ELISA Kit	IL-6 Gene Symbol: Il6 Il-6 Gene ID: 16193 Accession #: P08505 ELISA Kit: Mouse IL-6 ELISA Kit	IL-12p70 Gene Symbol: Il12a Gene ID: 16159 Accession #: P43431 ELISA Kit: Mouse IL-12 p70 ELISA Kit	TNF-α Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 21926 Accession #: P06804 ELISA Kit: Mouse TNF-alpha ELISA Kit	IFN-γ Gene Symbol: Ifng Gene ID: 15978 Accession #: P01580 ELISA Kit: Mouse IFN-gamma ELISA Kit
IL-10 Gene Symbol: Il10 Il-10 Gene ID: 16153 Accession #: P18893 ELISA Kit: Mouse IL-10 ELISA Kit	IL-1β Gene Symbol: Il1b Gene ID: 16176 Accession #: P10749 ELISA Kit: Mouse IL-1 beta ELISA Kit	?	?	?

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex? Magnetic Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^? Serum Diluent
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Flat-bottom 96-well Microplate

Additional Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 mL polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Automated plate washing system with magnetic separation
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading
Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex? bead arrays. Get a quote!

Assay Procedure Summary

Prepare Reagents, Standards, and Samples
Add MagPro RayPlex? Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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文件

FAMM-AV-1.pdf

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